Porphyromonas gingivalis is a bacterial pathogen that produces the polyproteins RgpA and Kgp, which are proteolytically processed into proteinases and adhesins. We have demonstrated that the RgpA and Kgp proteinases and adhesins are C terminally processed by carboxypeptidase CPG70 by sequencing C-terminal peptides from both the wild type and an isogenic CPG70 mutant, using ion trap mass spectro...

The receptor specificities of four Escherichia coli cloned hemagglutinins, AFA-I, AFA-III, F1845 fimbriae, and the Dr hemagglutinin were studied. Evidence is provided that all four hemagglutinins recognize as their receptor the Dr blood group antigen. However, results of experiments using enzyme-treated erythrocytes and monoclonal antibodies indicate that the four adhesins recognize different e...

Adhesion of Trichomonas vaginalis is believed to be dependent on four adhesion proteins, which are thought to bind to vaginal epithelial cells in a specific manner with a ligand-receptor type of interaction. However, the specific receptors on the host cell have not yet been identified. In this work, the ability of the T. vaginalis adhesins to bind to cells of different histologic derivations an...

MOTIVATION The adhesion of microbial pathogens to host cells is mediated by adhesins. Experimental methods used for characterizing adhesins are time-consuming and demand large resources. The availability of specialized software can rapidly aid experimenters in simplifying this problem. We have employed 105 compositional properties and artificial neural networks to develop SPAAN, which predicts ...

The ability of Escherichia coli which possess or lack mannose-sensitive adherence factors (adhesins) to associate with human peripheral leukocytes in vitro in the absence of serum was studied. E. coli 19+, which have mannose-sensitive adhesins, were derived from E. coli strain 19 by culturing in static Trypticase soy broth at 37 degrees C. E. coli 19-, which lack mannose-sensitive adhesins, wer...

background: the pathogenesis of nontypeable haemophilus influenzae (nthi) begins with adhesion to the rhinopharyngeal mucosa. almost 38-80% of nthi clinical isolates produce proteins that belong to the high molecular weight (hmw) family of adhesins, which are believed to facilitate colonization. methods: in the present study, the prevalence of hmwa, which encodes the hmw adhesin, was determined...

Cover figure: A stereo view of the surface of the GafD1-178 showing the bound GlcNAc (courtesy of Michael Merckel) CONTENTS LIST OF ORIGINAL PUBLICATIONS SUMMARY 1. INTRODUCTION 1 1.1. The diversity of bacterial adhesins 1 1.2. Bacterial adhesins mediate attachment to a variety of targets 2