OBJECTIVE Alpha-thalassemia is frequently encountered in eastern Saudi Arabia. We wanted to find out laboratory based incidence and laboratory features of Hemoglobin H disease in the Dammam region. METHODS We retrospectively analyzed the results of Hemoglobin electrophoresis carried out during the last 5 years in our laboratory. Hemoglobin electrophoresis was performed on cellulose acetate, p...

By MORRIS GOODMAN, PH.D.* AND DAN H. CAMPBELL, Pn.D. T HE DEMONSTRATION by Pauling et al.’ that sickle cell anemia hemoglobin has a slightly higher isoelectric point (0.2 pH unit) than normal human adult hemoglobin and the reports of differences in ability to form nematic liquid crystals2 and solubility differences in strong salt solutions3 suggest that differences might also exist in immunoche...

By MORRIS GOODMAN, PH.D.* AND DAN H. CAMPBELL, Pn.D. T HE DEMONSTRATION by Pauling et al.’ that sickle cell anemia hemoglobin has a slightly higher isoelectric point (0.2 pH unit) than normal human adult hemoglobin and the reports of differences in ability to form nematic liquid crystals2 and solubility differences in strong salt solutions3 suggest that differences might also exist in immunoche...

In murine erythroleukemia cells, the minor/major hemoglobin (Hb) ratio depends on the cell line and the inducing agent. To determine whether mouse minor hemoglobin is a "fetal" hemoglobin in vivo, globin chain composition and synthesis rates were determined in DBA/2 mice of various ages ranging from 14-day embryos to > 6-mo adults. Globin chains were separated by electrophoresis on polyacrylami...

The adult patients of tetralogy of Fallot often present with high hemoglobin levels. High hemoglobin and hematocrit on cardiopulmonary bypass (CPB) are associated with increased hemolysis, plasma free hemoglobin, renal dysfunction or failure, postoperative bleeding, exploration for bleeding, and increased requirement of allogeneic blood and blood products. Despite the presence of high hemoglobi...

Interferons have the ability to enhance or diminish the expression of specific genes and have been shown to affect the proliferation of certain cells. Here. the effect of ‘yinterferon on fetal hemoglobin synthesis by purified cord blood. fetal liver. and adult bone marrow erythroid progenitors was studied with a radioligand assay to measure hemoglobin production by BFU-E-derived erythroblasts. ...

1. Tadpole and adult hemoglobin do not differ significantly in molecular weight. The molecular weight of both is in the neighborhood of 68,000. 2. Heme-heme interaction as measured by the value of n in Hill's equation is virtually the same-about 2.8-in both tadpole and adult. 3. There appears to be no significant effect of pH upon the oxygen equilibrium of tadpole hemoglobin, in contrast to lar...