نتایج جستجو برای: affinity purification

تعداد نتایج: 136850  

Journal: :Journal of Biological Chemistry 1970

Journal: :Journal of Biological Chemistry 1998

Journal: :JOURNAL OF CHEMICAL ENGINEERING OF JAPAN 1993

Journal: :Proceedings of the National Academy of Sciences 1976

Journal: :Cold Spring Harbor Protocols 2011

Protein specific aptamers are highly applicable affinity ligands in different fields of research and clinical applications. They have been developed against various targets, in particular, bio-macromolecules such as proteins. Among human proteins, the coagulation factors are the most attractive targets for aptamer selection and their specific aptamers had valuable characteristics in therapeutic...

Protein specific aptamers are highly applicable affinity ligands in different fields of research and clinical applications. They have been developed against various targets, in particular, bio-macromolecules such as proteins. Among human proteins, the coagulation factors are the most attractive targets for aptamer selection and their specific aptamers had valuable characteristics in therapeutic...

Journal: :BioTechniques 2000
M Bergseid A R Baytan J P Wiley W M Ankener M L Stolowitz K A Hughes J D Chesnut

A new chemical affinity system is described for the purification of proteins. The Linx Affinity Purification System enables researchers to quickly and easily bind a protein ligand to a chromatographic matrix and use the resulting affinity resin to purify a second protein from crude mixtures. The entire process takes approximately 2 h.

Journal: :Eukaryotic cell 2010
Hui-Lin Liu Aysha H Osmani Leena Ukil Sunghun Son Sarine Markossian Kuo-Fang Shen Meera Govindaraghavan Archana Varadaraj Shahr B Hashmi Colin P De Souza Stephen A Osmani

A single-step protein affinity purification protocol using Aspergillus nidulans is described. Detailed protocols for cell breakage, affinity purification, and depending on the application, methods for protein release from affinity beads are provided. Examples defining the utility of the approaches, which should be widely applicable, are included.

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