BACKGROUND It has been suggested that the presence of Toxocara canis larvae in lungs is an underlying factor in the onset of asthma. Although the association of asthma and seropositivity to Toxocara has been observed, there are no studies that indicate whether these antibodies are specific against T. canis antigens. METHODS Seroprevalence to T. canis excretion-secretion antigens (TcES Ag) wer...

BACKGROUND Diagnosis of tuberculosis (TB) remains problematic despite many new advanced diagnostic methods. A reliable and rapid diagnostic test, which could be performed in any standard pathology laboratory, would help to obtain definitive early diagnoses of TB. In the present study we describe a prospective evaluation for demonstrating Antigen (Ag) 85 complex in the sera from TB patients. M...

BACKGROUND We compared the performance of two new commercial tests for the detection of dengue NS1 protein during the clinical phase of dengue virus (DENV) infection-an immunochromatographic test allowing rapid detection of the NS1 antigen, Dengue NS1 Ag STRIP (Bio-Rad Laboratories - Marnes La Coquette, France), and a two-step sandwich-format microplate enzyme-linked immunosorbent assay (ELISA)...

Introduction: It is estimated that one-third of the world’s population is infected with M. tuberculosis. Also we came to know that the numbers of TB cases are on the rise in our rural based tertiary care hospital. We searched for Mycobacterium tuberculosis antigen in the serum of patients with pulmonary TB by developing Sandwich ELISA. Materials & Methods: We took 50 pulmonary TB patients, 50 d...

To determine the prevalence of porcine cysticercosis, a survey was carried out in 27 villages belonging to two rural communities of West-Cameroon (Bafou and Bamendou). Between January and August, 2000, a total of 707 pigs were examined serologically and by tongue inspection. Serum samples were examined for circulating parasite antigen using a monoclonal antibody-based sandwich enzyme-linked-imm...

In this study, AIV (AIV) Ag of A type with viral nucleoprotein (NP), which covers all AIV subtypes, including high-pathogenic AIV, was demonstrated by the novel format of polystyrene microfluidic chip ELISA. In micro-channels of chip instead of conventional 96-well microtiter plates, a double-antibody sandwich ELISA (DAS-ELISA) was developed, in which rabbit Ab, mouse nuclear protein (NP) mAb a...

BACKGROUND Taenia solium taeniosis/cysticercosis is a parasitic infection occurring in many developing countries. Data on the status of human infections in Zambia is largely lacking. We conducted a community-based study in Eastern Zambia to determine the prevalence of human taeniosis and cysticercosis in a rural community. METHODS AND FINDINGS Stool and serum samples were collected from willi...

Diagnostic systems for Lassa fever (LF), a viral hemorrhagic fever caused by Lassa virus (LASV), such as enzyme immunoassays for the detection of LASV antibodies and LASV antigens, were developed using the recombinant nucleoprotein (rNP) of LASV (LASV-rNP). The LASV-rNP was expressed in a recombinant baculovirus system. LASV-rNP was used as an antigen in the detection of LASV-antibodies and as ...