BACKGROUND Antimicrobial susceptibility testing (AST) of Clostridium difficile is increasingly important because of the rise in resistant strains. The standard medium for the AST of C. difficile is supplemented Brucella agar (sBA), but we found that the growth of C. difficile on sBA was not optimal. Because active growth is critical for reliable AST, we developed a new, modified C. difficile (m...

backgrounds: to obtain information about clinical isolates of aspergillus flavus group. methods: we examined 55 isolates [45 clinical, 10 reference (6 from culture collections, 4 local reference)] for toxicology, growth rates, and morphological and physiological characteristics. modified czapek agar (cz) and malt extract agar (mea) were used for observing microscopic morphology and measuring fu...

A medium, lysine-arginine-iron agar, was developed for the presumptive identification of Yersinia enterocolitica isolates. This medium was a modification of lysine-iron agar and allowed for the testing of five biochemical characteristics in a single tube medium. The reactions of Y. enterocolitica on this medium were reliable and distinctive. The medium significantly simplified the identificatio...

AIMS To evaluate the sensitivity and specificity of two selective media for the isolation of Burkholderia cepacia from sputum specimens in patients with cystic fibrosis (CF). METHODS In total, 149 expectorated sputum specimens from 113 patients with CF (32 cepacia colonised patients and 81 non-cepacia colonised patients) attending three CF centres were examined for the presence of B cepacia o...

The aim of this study was to identify aflatoxin producing fungi from affected corn and peanut samples collected from the storage godowns. Fungi were isolated from corn and peanuts using potato dextrose agar and modified rose bengal agar. The pure culture of the isolates were grown on differential media namely malt extract agar, czapek yeast extract agar and czapek dox agar for species identific...

The purpose of this study is to develop a selective and differential medium (SK2 agar) for isolating guaiacol producing Alicyclobacillus. Forty-one selected dyes and vanillic acid were incorporated in SK agar for screening selective and differential agents. Two guaiacol producing (1016, 1101) and two non-guaiacol producing (19220, C-GD 1-1) Alicyclobacillus isolates were streaked onto media and...

A new medium for the isolation of Pseudomonas cepacia from respiratory tract secretions of patients with cystic fibrosis (CF) is described. This medium consists of inorganic salts, 0.5% pyruvate, and 0.1% proteose peptone as nutritive components and 0.0001% crystal violet, 0.15% bile salts, 100 micrograms of ticarcillin per ml, and 300 U of polymyxin B per ml as selective agents. The medium, de...