Besides their traditional food usage, mushrooms can be used as a source of many pharmacologically active compounds, especially polysaccharides. Wastes left over in the edible mushroom farms such as Agaricus bisporus (white button mushroom) can also become free and alternative source of chitin-chitosan materials, beside the traditional industrial source shellfish waste materials. Chitosan, a nat...

The cultivated mushroom of commerce in the Western hemisphere, Agaricus bisporus, was given p.o. to randomly bred Swiss mice for 3 days and was followed by semisynthetic diet for 4 days each week for life. The mice were 6 weeks old at the beginning of the experiment. As a result of treatment, tumors were induced in the bone, forestomach, liver, and lungs in the following incidences: 16, 38, 8, ...

background and purpose: although molds are regarded as the main fungal allergen sources, evidence indicates that spores of basidiomycota including agaricus bisporus (a. bisporus) can be also found at high concentrations in the environment and may cause as many respiratory allergies as molds. the aim of the present study was to evaluate specific immunoglobulin e (ige) and immunoglobulin g (igg) ...

BACKGROUND The differentiation resp. death of human monocytic THP-1 cells induced by polysaccharide extracts of the medicinal mushrooms Phellinus linteus, Agaricus bisporus and Agaricus brasiliensis have been studied. This study aims to identify leads for the causal effects of these mushroom components on cell differentiation and death. METHODS THP-1 cells were treated with different polysacc...

The antioxidant action of ethanolic extract from Agaricus bisporus which is available in the supermarkets of Bucharest, Romania, was assessed by determining its reducing power and its radical scavenging activity. The determinations were made by analyzing the freeze-dried ethanolic extract. The radical scavenging activity was determined using 2, 2-diphenyl-1-picrylhydrazyl (DPPH), superoxide rad...

Agaricus bisporus is a secondary decomposer fungus and an excellent model for the adaptation, persistence and growth of fungi in humic-rich environments such as soils of temperate woodland and pastures. The A. bisporus serine proteinase SPR1 is induced by humic acids and is highly expressed during growth on compost. Three Spr1 gene silencing cassettes were constructed around sense, antisense an...

Pseudomonas tolaasii, the causative agent of Agaricus bisporus brown blotch disease, can be identified by the white line reaction, occurring upon confrontation of the tolaasin-producing mushroom pathogen with "Pseudomonas reactans," producing the lipopeptide white line-inducing principle (WLIP). The draft genome sequence of the WLIP-producing indicator Pseudomonas fluorescens strain LMG 5329 is...

Wild and cultivated Basidiomycetes species were cultured to determine the distribution of bacteria causing brown blotch disease of Agaricus bisporus. Colonies from each basidiocarp were screened for brown blotch organisms by the white line and host pathogenicity tests. Isolates causing brown blotch were identified as Pseudomonas tolaasi and an Arthrobacter species.

We describe a modified Agrobacterium-mediated method for the efficient transformation of Agaricus bisporus. Salient features of this procedure include cocultivation of Agrobacterium and fruiting body gill tissue and use of a vector with a homologous promoter. This method offers new prospects for the genetic manipulation of this commercially important mushroom species.