BACKGROUND Argyrophilic nucleolar organizer regions (AgNORs) and MIB-1 as proliferating activities have been applied separately to assess the malignant potential of cancer cells. We conducted staining of AgNORs and MIB-1 in 42 surgically-resected invasive breast carcinomas. MATERIALS AND METHODS Paraffin-embedded sections were used for double staining and the mean AgNOR counts in 100 MIB-1-po...

Objective: To evaluate the correlation of argyrophilic nucleolar regions(AgNORs) and malignancy in benign and malignant effusions. Design: The study group consisted of pleural and peritoneal effusion samples obtained from patients suffering from various benign or malignant diseases. The cytological smears were studied by conventional haematoxylin & eosin(H&E) and silver staining for AgNORs. Set...

AIM To study the Argyrophilic Nucleolar Organizer Regions (AgNOR) as a cell proliferative marker in cervical smears and to assess its counts with the severity of the lesions on Papanicolaou (PAP) stained cervical smears. MATERIAL AND METHOD The present study was a prospective experimental study which was carried out at Jawaharlal Nehru Medical College in a span of 2 years. 50 high risk cases ...

Background: Nucleolar organizing regions (NORs) are associated with proliferative activity and represents as a diagnostic aid in several neoplastic entities. Objective: The purpose of this study was to determine and compare the AgNOR (Silver binding nucleolar organizing region) count in healthy oral mucosa, oral leukoplakia and oral squamous cell carcinoma (OSCC). Methods: The biopsied tissue s...

OBJECTIVE To differentiate between reactive plasmacytosis (RP) and multiple myeloma (MM) in bone marrow aspirates by assessment of silver-stainable nucleolar organizer regions (AgNORs). METHODS Archival cytologic smears (n = 69) from bone marrow aspirates were retrospectively investigated. Twenty cases had RP and 49 cases MM (24 low-grade, 22 intermediate-grade and 3 high-grade MM). Diagnoses...

BACKGROUND The relationship between the population doubling time and the quantity of silver-stained nucleolar organizer region (AgNOR) interphase proteins was studied in cell culture at three different temperatures used to modulate the cell cycle duration. METHODS After MIB 1 and AgNOR combined staining, the quantity of AgNOR proteins was measured in cycling cells by image cytometry. RESULT...

Nucleolar organising regions (NOR) are part of the nucleolus, containing argyrophilic proteins (nucleoclin/C23, nucleophosmin/B23). They are identified by silver staining at low pH. The method also reveals osteocyte canaliculi and cement lines and granules in the cytoplasm of kidney cells in locations that mimic osteopontin distribution. Human bone and kidney sections, benign and lymphomatous p...

The selective staining of nucleolar acidic proteins by silver dyes (AgNOR technique) is a promising tool to discriminate malignant cells from benign cells in tumour pathology. Until now, the existing methods to measure the distribution of the AgNOR proteins were performed in two-dimensional space. Confocal scanning laser microscopy (CSLM) and progress in computer sciences make it possible to im...

The aim of this study was to evaluate the diagnostic value of AgNOR (Argyrophilic Nucleolar Organizer Proteins Region) staining in differentiation between malignant and non-malignant lesion, and histological grading of bladder transitional cell carcinoma. We have also showed the rate of AgNOR distribution score in the nuclear of malignant and non-neoplastic cells of bladder epithelium as an imp...

BACKGROUND Serous effusion smears reported as (suspicious for malignancy) pose problems in clinical management. Silver staining for argyrophilic nucleolar organizer regions (AgNOR) has proved useful in making a cytopathologic differential diagnosis between benign and malignant cells. Nucleolar organizer regions(NORs) are loops of DNA located in acrocentric chromosomes. These NORs are visualized...