During antidiuresis, renal papillary cells accumulate organic osmolytes preferentially over inorganic ions. It has been previously demonstrated that sodium infusion increased all of these organic osmolytes except myo-inositol (1). Conversely, urea infusion increased only glycerophosphorylcholine significantly. In addition to sodium and urea, potassium localized in tissue and urine influenced th...

Naphthalene feeding can result in cataract formation in rats and rabbits due to specific metabolites of naphthalene. The concomitant administration of the aldose reductase inhibitor Al1576 to naphthalene-fed rats was proven to prevent cataract formation. To determine whether this effect was directly linked to the ability of Al1576 to inhibit enzyme aldose reductase, a variety of structurally di...

background: the relationship between the high activity of aldose reductase (ar) and diabetic cataract formation has been previously investigated. the purpose of the present study was to determine the preventing effect of melatonin on streptozotocin (stz)-induced diabetic cataract in rats. methods: 34 adult healthy male sprague-dawely rats were divided into four groups. diabetic control and diab...

In vitro antidiabetic effect of fruits and vegetables with reports as folk remedies were investigated. The antidiabetic effects were evaluated by comparing the inhibitory properties of α-glycosidase, aldose reductase, and antioxidant activity. The results indicated that lychee extract exhibited the best dose-dependent inhibitory activity against α-glycosidase with IC 50 of 10.4 mg/mL, and lemon...

A monomeric (Mr = 43,000) NADPH-dependent oxidoreductase with a broad substrate specificity for aliphatic and aromatic aldehydes and aldo sugars has been purified from the skeletal muscle of female and castrated pigs. The properties of this enzyme are consistent with those of aldose reductase (EC 1.1.1.21), and the enzyme is immunologically identical with aldose reductase from pig lens. However...

The sequence of human foetal liver aldose reductase cDNA obtained from a \-gtlO library 1s presented. This cDNA encodes a protein of 316 ami no adds with a 45 bp 5'-non coding region and a 356 bp 3 1 non coding region. Aldose reductase Is of wide clinical Interest 1n that Inhibitors may prevent the complications associated with diabetes melHtus. The cDNA clone was Identified using ol1gonucleot1...

Aldose reductase has been implicated in the etiology of diabetic complications. A variety of compounds have been observed to inhibit aldose reductase and effective, orally active inhibitors of the enzyme have been investigated for many years. Although several of these compounds have progressed to the clinical level, only one such drug is currently on the market. Due to the limited number of ava...

Aldose reductase (alditol:NADP+ 1-oxidoreductase, EC 1.1.1.21) has been partially purified from human erythrocytes by DEAE-cellulose (DE-52) column chromatography. This enzyme is activated severalfold upon incubation with 10 microM each glucose 6-phosphate, NADPH, and glucose. The activation of the enzyme was confirmed by following the oxidation of NADPH as well as the formation of sorbitol wit...

PURPOSE Increased flux of glucose via the polyol pathway, oxidative stress and ischaemia lead to the upregulation of the aldose reductase (AR), the key enzyme of the polyol pathway. This adversely affects the organism and can in part be reduced by inhibition of the enzyme. METHODS In this study, we examined the effect of the HMG-CoA-reductase inhibitor atorvastatin on the expression of aldose...

In an effort to characterize active principles for diabetic complication from medicinal mushroom, aldose reductase inhibitors were isolated from the fruiting body of Phellinus linteus and identified as hispidin (5), phelligridimer A (6), davallialactone (7), methyldavallialactone (8), hypholomine B (9), interfungins A (10), and inoscavin A (11), together with protocatechuic acid (1), protocatec...