The structural phenotype of the movement proteins (MPs) of two representatives of the Bromoviridae, alfalfa mosaic virus (AMV) and brome mosaic virus (BMV), was studied in protoplasts. Immunofluorescence microscopy showed that the MPs of these viruses, for which there has been no evidence of a tubule-guided mechanism, assemble into long tubular structures at the surface of the infected protopla...

Four insects species have emerged as important pests of alfalfa in the Western United States during the past decade. Cowpea aphid has long been a resident insect of the Western United States, but became a significant alfalfa pest during the past two years. The silverleaf whitefly has been a serious pest of alfalfa since 1991. Silverleaf whitefly reduces hay yield and contaminates alfalfa hay wi...

Net release of degraded N as NH3 and total AA plus microbial protein synthesis, quantified from incorporation of 15NH3 into microbial protein, was used to estimate the rate and extent of in vitro degradation of protein fractions isolated from alfalfa hay and silage. Seven proteins (casein, alfalfa hay, alfalfa silage, extracts from alfalfa hay and silage, and residues from alfalfa hay and silag...

Current Alfalfa Cultivation and Utilization ........................................................80 Development and Cultivation of Alfalfa for Biomass........................................82 Chemical Composition of Alfalfa.......................................................................83 Genetic Impacts on Composition ....................................................................

Value-added traits of alfalfa are needed to provide farmers new high value profitable products. Processing alfalfa to obtain value added products includes three different fractionation methods: 1) wet fractionation; separation into juice fraction and a fiber fraction, 2) dry fractionation; separation into leaves and stems, and 3) fractionation by passage of the whole herbage through the digesti...

The minus-strand promoter of Alfalfa mosaic virus (AMV), a tripartite plant virus belonging to the family Bromoviridae, is located within the 3'-terminal 145 nucleotides (nt), which can adopt a tRNA-like structure (TLS). This contrasts with the subgenomic promoter for RNA4 synthesis, which requires approximately 40 nt and forms a single triloop hairpin. Detailed analysis of the minus-strand pro...

N6-methyladenosine (m6A) is an internal, reversible nucleotide modification that constitutes an important regulatory mechanism in RNA biology. Unlike mammals and yeast, no component of the m6A cellular machinery has been described in plants at present. m6A has been identified in the genomic RNAs of diverse mammalian viruses and, additionally, viral infection was found to be modulated by the abu...

Alfalfa hay utilization by dairy cattle has expanded in western dairy states. Alfalfa silage utilization has increased in the Midwest. Alfalfa haylage contains high levels of nonprotein nitrogen (NPN) due to protein breakdown during fermentation. Excessive levels of NPN in alfalfa silage diets require supplementation of protected protein sources to meet protein demands of high-producing cattle....

The Egyptian alfalfa weevil (alfalfa weevil in some areas) during most years is the most severe insect pest of alfalfa in California. These pests feed on alfalfa leaflets and stems, reducing hay yields. Several insecticides provide acceptable control of this pest. Historically, product choice has been driven by efficacy on the pest, cost, presence of other pests, i.e., aphids, days before harve...

Alfalfa (Medicago sativa L.) is an important legume forage crop with great economic value. However, as the growth of alfalfa is seriously affected by an inadequate supply of water, drought is probably the major abiotic environmental factor that most severely affects alfalfa production worldwide. In an effort to enhance alfalfa drought tolerance, we transformed the Arabidopsis Enhanced Drought T...