BACKGROUND Embryonated chicken eggs have been used since the mid-20th century to grow a wide range of animal viruses to high titers. However, eggs have found so far only limited use in the production of recombinant proteins. We now describe a system, based on a Sendai virus minigenome, to produce large amounts of heterologous viral glycoproteins in the allantoic cavity of embryonated eggs. RE...

Chick embryos infected intra-allantoically with nonvirulent staphylococci are protected from death due to infection with virulent staphylococci. The protection is associated with a delay in growth of the challenge strain and a delay in the production of toxic substances in the allantoic fluid. The protection is influenced by the number of bacteria in the protecting and challenge inocula and by ...

After allantoic injection of chick embryos with a known amount of influenza virus, the process of adsorption of the agent onto host cells and infection of them can be interrupted at a given time by the administration of large quantities of heterologous virus inactivated by irradiation. A sudden great increase in the amount of free virus in the allantoic fluid occurring after 6 hours in the case...

Subcutaneous inoculation, of PR8 allantoic fluid, or watery suspensions of the virus obtained from allantoic fluid by high-speed centrifugation or by elution after adsorption on red cells induced serum antibodies in experimental animals, which reached the highest levels within 2 weeks after inoculation and were gradually lost thereafter. The addition of killed acid-fast bacteria (Myco. tubercul...

Two oil emuisified vaccines of Newcaslte disease were prepared from Lasota strain. The virus was propagated in 9-day-old chicken embryos through allantoic route. The haemagglutination titre of the allantoic fluid used for vaccine preparation was 2048. The fluid was inactivated by 0.1 per cent f nal concentration of formalin at 37 oc for 6 hours. Inactivated allantoic fluid was emulsified with a...

  Egg drop syndrome (EDS) is caused by a hemagglutinating adenovirus which has become a major cause of lost egg production and sever economic losses in breeder and layer chicken flocks throughout the world. A PCR assay was optimized for detection of EDS virus in inoculated allantoic fluids (AFs) of duck and chicken emberyonated eggs. Two strains of EDS viruses were propagated in allantoic cavit...