Previous results indicated that performance of chicks fed a high level (10%) of poultry by-product meal(PBM) was lower than performance of chicks fed a corn-soybean meal (SBM) diet. The latter difference was hypothesized to be due to variation in digestible amino acid (AA) levels among the diets. This study evaluated diets containing PBM formulated on equivalent total AA basis vs. an equivalent...

Amyloid protein AA is the presumptive fragment of an acute phase serum apolipoprotein, apoSAA. Two major murine apoSAA isotypes (apoSAA1 and apoSAA2) have been identified. The NH2-terminal amino acid sequences of purified murine apoSAA1 and apoSAA2 have been examined and compared with that of murine amyloid protein AA. Our results indicate that apoSAA1 and apoSAA2 are separate gene products and...

Maternal high-protein supplements designed to increase birth weight have not been successful. We recently showed that maternal amino acid infusion into pregnant sheep resulted in competitive inhibition of amino acid transport across the placenta and did not increase fetal protein accretion rates. To bypass placental transport, singleton fetal sheep were intravenously infused with an amino acid ...

Preimplantation mouse embryos exhibit growth at the blastocyst stage when there is a dramatic increase in protein content between days 4 and 5 after hCG treatment. This increse in protein synthesis requires the uptake of amino acids from the surrounding medium, but the consumption of individual amino acids present as a mixture has not been examined. We therefore measured the simultaneous deplet...

780 amino acids (aa), 86.13 KDa (isoform I) and 731 amino acids, 80.76 KDa (isoform II). Striatin contains a high number of domains mediating protein-protein interactions. Domains: aa 55-63 : Caveolin-binding; aa 53-120 : Coiled-coil; aa 149-166 : Calmodulin-binding; aa 461-500 : WD 1; aa 514-553 : WD 2; aa 567-606 : WD 3; aa 662-701 : WD 4; aa 704-743 : WD 5; aa 750-779 : WD 6 (30 aa only) (Is...

The ancient essential process of ribosomal protein synthesis requires twenty sets of aminoacyl-tRNAs (aa-tRNAs), one for each canonical amino acid. Since Crick proposed his adaptor hypothesis (1) it was commonly accepted that all organisms possess twenty aaRSs, each enzyme specific for attaching one amino acid to tRNA. It is now clear that aa-tRNA formation is more varied, as the biosynthetic r...

Amino acids and glucose acutely stimulate fetal insulin secretion. In isolated adult pancreatic islets, amino acids potentiate glucose-stimulated insulin secretion (GSIS), but whether amino acids have this same effect in the fetus is unknown. Therefore, we tested the effects of increased fetal amino acid supply on GSIS and morphology of the pancreas. We hypothesized that increasing fetal amino ...

We studied the effect of an acute 4-h period of hyperinsulinemia (H) on net utilization rates (AAUR(net)) of 21 amino acids (AA) in 17 studies performed in 13 late-gestation fetal sheep by use of a novel fetal hyperinsulinemic-euglycemic-euaminoacidemic clamp. During H [84 +/- 12 (SE) microU/ml H, 15 +/- 2 microU/ml control (C), P < 0. 00001], euglycemia was maintained by glucose clamp (19 +/- ...