Acidosis and angiotensin II stimulate ammonia production and transport by the proximal tubule. We examined the modulatory effect of the type 1 angiotensin II receptor blocker losartan on the ability of metabolic acidosis to stimulate ammonia production and secretion by mouse S2 proximal tubule segments. Mice given NH(4)Cl for 7 days developed metabolic acidosis (low serum bicarbonate concentrat...

In larval Aedes aegypti, transcripts of the Rhesus-like glycoproteins AeRh50-1 and AeRh50-2 have been detected in the anal papillae, sites of ammonia (NH3/NH4+) excretion; however, these putative ammonia transporters have not been previously localized or functionally characterized. In this study, we show that the AeRh50s co-immunolocalize with apical V-type H+-ATPase as well as with basal Na+/K...

The use of air cleaning systems to reduce ammonia emissions from animal houses is increasing. These systems are normally used in order to comply with local or national regulations of ammonia emission. Therefore, accurate determination of the proportion of ammonia being removed by these systems is crucial. There are two main methods available to measure ammonia removal efficiency of scrubbers: a...

Renal ammonia metabolism is a fundamental element of acid-base homeostasis, comprising a major component of both basal and physiologically altered renal net acid excretion. Over the past several years, a fundamental change in our understanding of the mechanisms of renal epithelial cell ammonia transport has occurred, replacing the previous model which was based upon diffusion equilibrium for NH...

Concentrated animal feeding operations are major sources of ammonia emitted to the atmosphere. There is a considerable literature on ammonia emissions from poultry and swine, but few studies have investigated large, open lot beef cattle feedyards. We used the micrometeorological fluxgradient method to estimate ammonia emissions during six field campaigns in three seasons. Profiles of ammonia, w...

Ammonia has been shown to function as a morphogen at multiple steps during the development of the cellular slime mold Dictyostelium discoideum; however, it is largely unknown how intracellular ammonia levels are controlled. In the Dictyostelium genome, there are five genes that encode putative ammonium transporters: amtA, amtB, amtC, rhgA, and rhgB. Here, we show that AmtA regulates ammonia hom...

Ammonia (NH3) emissions originate predominantly from manure. In outdoor pig production, excretory behaviour creates nitrogen (N) point loads. This study examined ammonia losses from pigs on grassland at two farms by calculating N field balances per pen and sub-areas preferred (P) and not preferred (NP) for excretion. Spatial variation in ammonia losses was measured with an equilibrium concentra...

A novel graphene-coated Ni electrode was developed in this investigation to improve corrosion resistance while unexpectedly enhancing the ammonia generation rate in the electrochemically induced urea to ammonia (eU2A) process, which is an electrochemical onsite ammonia generation method. The development of the electrode is crucial for the eU2A reactions since in the ammonia generation process, ...