We evaluated four kinetic amylase procedures with respect to kinetics, analytical range, blank rates, reagent stability, reagent impurities, interfering substances, and intrinsic sensitivities. Each of the methods is shown to have its own unique advantages and disadvantages. A preliminary discussion of some alternative methods, in which glycosidic p-nitrophenyl alpha-oligosaccharides are substr...

elm leaf beetle, xanthogaleruca luteola, is a serious pest (particularly at its larval stage) of elm trees in many areas. the existence of three important enzyms namely: ?-amylase, ?- glucosidase and ?-glucosidase in the midgut of the insect at its larval stage was investigated. optimum activity for ?-, ?-glucosidase and ?-amylase was recorded at phs 5.0, 6.0 and 6.0 against p-nitrophenyl-?-d-g...

background: a-amylases (ec 3.2.1.1) are covering approximately 25% of total enzyme market and are frequently used in food, pharmaceutical and detergent industries.objectives: the first ever detailed characterization of amylase from any halophilic engyodontium album is presented. materials and methods: an extracellular α-amylase was studied from halophilic e. album tistr 3645. the enzyme was ext...

Th e paper presents numerical comparison of two methods of estimation of nonallelic interaction of QTL eff ects. In the fi rst method we assume that we observe only the plant phenotype, while in the second method we have additional information from the molecular markers observations. In this paper we analysed phenotypic data on 120 barley doubled haploid lines, derived from cross Clipper × Saha...

diabetes mellitus is one of the most common endocrine diseases and its type ii is the major form of diabetes, accounting for 90% of cases worldwide. the inhibition of carbohydrate hydrolyzing enzymes such as ?-amylase can be an important strategy in the control of blood glucose levels in patients with type ii diabetes. in this investigation, three morus species, m. alba, m. alba var. nigra and ...

Background: a-Amylases (EC 3.2.1.1) are covering approximately 25% of total enzyme market and are frequently used in food, pharmaceutical and detergent industries. Objectives: The first ever detailed characterization of amylase from any halophilic Engyodontium album is presented. Materials and Methods: An extracellular α-amylase was studied from halophilic E. album TISTR 3645. The enzyme was e...

objective(s):one strategy for the treatment of diabetes is inhibition of pancreatic α- amylase. plants contains different chemical constituents with potential for inhibition of α-amylase and hence maybe used as therapeutic. materials and methods: urtica dioica and juglans regia linn were tested for α-amylase inhibition. different concentrations of leaf aqueous extracts were incubated with enzym...

Porcine pancreatic alpha-amylase (PPA) was allowed to react with herbal extracts containing rosmarinic acid (RA) and purified RA. The derivatized enzyme-phytochemical mixtures obtained were characterized for residual amylase activity. These in vitro experiments showed that the amylase activity was inhibited in the presence of these phytochemicals. The extent of amylase inhibition correlated wit...