Amylolytic enzymes represent a group of starch hydrolases and related enzymes that are active towards the α-glycosidic bonds in starch and related polyand oligosaccharides. The three best known amylolytic enzymes are α-amylase, β-amylase and glucoamylase that, however, differ from each other by their amino acid sequences, three-dimensional structures, reaction mechanisms and catalytic machineri...

A continuous research is attempted to fulfil the highest industrial demands of natural amylases presenting special properties. New α-amylases extracted from stems and leaves of Pergularia tomentosa, which is widespread and growing spontaneously in Tunisia, were studied by the means of their activities optimization and purification. Some similarities were recorded for the two identified enzymes:...

This review makes a comprehensive survey of microbial amylases, i.e. alpha-amylase, beta-amylase and glucoamylase. Amylases are among the most important enzymes and are of great significance in present-day biotechnology. Although they can be derived from several sources, such as plants, animals and micro-organisms, the enzymes from microbial sources generally meet industrial demands. Microbial ...

Foreign amylase addition to honey in an effort disguise diastase activity has become a widespread form of food fraud. However, since there is no report on the investigation Japan, we investigated foreign amylases 67 commercial honeys Japan. First, α-glucosidase and activities were measured, which revealed that only was significantly low several samples. As both enzymes are secreted from honeybe...

Bacterial amylase from Bacillus subtilis and fungal amylase from Aspergillus niger were potential source for industrial exploitation in the field of amylase production. Hence, this study on bacterial amylase and fungal amylase was done to compare the kinetic parameter of both the types of amylases. 30-60% ammonium sulfate precipitation has done which showed 90 % of purification of both the part...

K-Amylases constitute a very diverse family of glycosyl hydrolases that cleave K1C4 linkages in amylose and related polymers. Recent structural and mutagenic studies of archeael, mammalian and bacterial K-amylases have resulted in a wealth of information on the catalytic mechanism and on the structural features of this enzyme class. Because of their high thermo-stability, the Bacillus K-amylase...

Amylases are important industrial enzymes that have wide applications ranging from conversion of starch to sugar syrups, the production cyclodextrins for pharmaceutical industry. This investigation aimed at amylases using Aspergillus and Fusarium species isolated waste-corncobs in Keffi Nigeria. Standard microbiological methods were employed isolation identification fungal isolates. The yields ...

We investigated the behavior of 26 quality-control sera with the inhibitor method for differential amylase (EC 3.2.1.1) assay. We also studied the sensitivity to the wheat-derived inhibitor of pancreatic amylases from 10 different animals in comparison with human pancreatic and salivary amylase. The results indicate that only control materials containing human amylases can be measured accuratel...

Amylases from incubated aleurone layers or from starchy endosperm of barley seeds (Hordeum vulgare L. cv. Himalaya) were investigated using acrylamide gel electrophoresis and analytical gel filtration with Sephadex G-200. Electrophoresis of amylase from aleurone layers yields seven visually distinct isozymes with an estimated molecular weight of 43,000. Because each isozyme hydrolyzes beta-limi...

Amino acid sequence comparison of 37 alpha-amylases from microbial, plant and animal sources was performed to identify their mutual sequence similarities in addition to the five already described conserved regions. These sequence regions were examined from structure/function and evolutionary perspectives. An unrooted evolutionary tree of alpha-amylases was constructed on a subset of 55 residues...