Extraction of sugar is the rate-limiting step in converting unpretreated biomass into value-added products through microbial fermentation. Both anaerobic fungi and anaerobic bacteria have evolved to produce large multi-cellulase complexes referred to as cellulosomes, which are powerful machines for biomass deconstruction. Characterization of bacterial cellulosomes has inspired synthetic "design...

The internal transcribed spacer (ITS) is a popular barcode marker for fungi and in particular the ITS1 has been widely used for the anaerobic fungi (phylum Neocallimastigomycota). A good number of validated reference sequences of isolates as well as a large number of environmental sequences are available in public databases. Its highly variable nature predisposes the ITS1 for low level phylogen...

The microeukaryotic community in Zodletone Spring, a predominantly anaerobic sulfide and sulfur-rich spring, was examined using an 18S rRNA gene cloning and sequencing approach. The majority of the 288 clones sequenced from three different locations at Zodletone Spring belonged to the Stramenopiles, Alveolata, and Fungi, with members of the phylum Cercozoa, order Diplomonadida, and family Jakob...

this study investigated the microbial community in a full scale anaerobic baffled reactor and sequencing batch reactor system for oil-produced water treatment in summer and winter. the community structures of fungi and bacteria were analyzed through polymerase chain reaction–denaturing gradient gel electrophoresis and illumina high-throughput sequencing, respectively. chemical oxygen demand eff...

Specimens from the conjunctival sacs of 92 healthy eyes were cultured on two separate occasions to determine the presence or absence of a persistent anaerobic flora. Aerobic bacteria and fungi were also studied for comparison. Of the 184 eye cultures, 112 (60.9%) contained at least one microorganism. Obligate anaerobes were recovered from 51.6% (95/184) of the cultures. Propionibacterium acnes,...

Eukaryotic communities in aerobic wastewater treatment processes are well characterized, but little is known about them in anaerobic processes. In this study, abundance, diversity and morphology of eukaryotes in anaerobic sludge digesters were investigated by quantitative real-time PCR (qPCR), 18S rRNA gene clone library construction and catalyzed reporter deposition-fluorescence in situ hybrid...

BACKGROUND Engineered cell factories that convert biomass into value-added compounds are emerging as a timely alternative to petroleum-based industries. Although often overlooked, integral membrane proteins such as solute transporters are pivotal for engineering efficient microbial chassis. Anaerobic gut fungi, adapted to degrade raw plant biomass in the intestines of herbivores, are a potentia...

This study was conducted to compare digestibility of wheat straw (WS) by fungi and whole rumen microorganisms (WRM). Dry matter (DM), neutral and acid detergent fiber (NDF and ADF) digestibility of WS were compared with in vitro digestion (IVD), gas production (GP) and specific rumen anaerobic fungi culture (SRAFC). Dry matter, NDF and ADF digestibility of WS by WRM of buffalo (60.80, 49.93 and...