BACKGROUND AND AIMS Understanding the fate and dynamics of cells during callus formation is essential to understanding totipotency and the mechanisms of somatic embryogenesis. Here, the fate of leaf explant cells during the development of embryogenic callus was investigated in the model legume Medicago truncatula. METHODS Callus development was examined from cultured leaf explants of the high...

Reciprocal differences persist in nature because of the unequal contribution of cytoplasmic determinants from male and female gametes to the zygote. The inheritance of genetic differences is an important factor that influences various traits, including somatic embryogenesis and regeneration in vitro. In this report, we estimate the cytoplasmic and maternal effects in pearl millet and their adeq...

Callus cultures from root explants of Lathyrus sativus L. ‘Derek’ were tested for their morphogenic capacity. Primary explants (fragments of roots) were cultivated on three induction media. We obtained three lines of callus tissue among which we identified two non-embryogenic lines and one embryogenic line. Callus originally cultivated on modified MS medium supplemented with 0.05 mgL picloram, ...

BACKGROUND The threatened plant Centella asiatica L. is traditionallyused for a number of remedies. In vitro plant propagation and enhanced metabolite production of active metabolites through biotechnological approaches has gained attention in recent years. RESULTS Present study reveals that 6-benzyladenine (BA) either alone or in combination with 1-naphthalene acetic acid (NAA) supplemented ...

Orchardgrass is a member of the Poaceae, a family characterized by monocotyledonous-type embryos. This species is unique in that somatic embryogenesis can be induced from the cultured leaves of potted plants, which can be maintained conveniently in the greenhouse. Both embryogenic callus and isolated somatic embryos develop directly from cultured leaf sections. Embryogenic callus can be maintai...

A cDNA named CsHPt1 (for Citrus sinensis histidine phosphotransmitter protein 1) containing a conserved domain characteristic of a twocomponent phosphorelay intermediate associated with signal transduction mechanisms, was isolated from globular embryos of C. sinensis sweet orange cv. ‘Valencia’ by differential display RT-PCR. The corresponding full-length cDNA, subsequently obtained by RACE-PCR...

Embryogenic callus culture of carrot (Daucus carota L.) was induced from the hypocotyl explants in MS medium supplemented with Casein hydrolysate, 2,4-D and BAP for somatic embryogenesis In vitro cell suspension cultures for high multiplication rate. Embryogenic callus was transferred to liquid basal Murashige and Skoog medium The mature somatic embryos were encapsulated in a Sodium alginate mi...

In present report effects of explants type, basal media and plant growth regulators (PGRs) were tested for induction of indirect somatic embryogenesis in medicinally important plant Silybum marianum L. Leaf, petiole and root explants were exploited in vitro on B5 (Gamborg), SH (Schenk and Hildebrandt) and MS (Murashige and Skoog) media for induction of embryogenic callus followed by somatic emb...

genus onobrychis is an important forage crop consisting of approximately 130 annual and perennial species. the present research focused on the cytological studies of embryogenic (e) and non-embryogenic (ne) callus as well as root meristem cells of in vitro and in vivo grown plants. on the contrary to the previous studies, a diploid viciifolia variety with 2n= 2x= 16 chromosomes was observed for...