The extracellular colorants produced by Chlorociboria aeruginosa, Scytalidium cuboideum, and Scytalidium ganodermophthorum, three commonly utilized spalting fungi, were tested against a standard woodworker's aniline dye to determine if the fungal colorants could be utilized in an effort to find a naturally occurring replacement for the synthetic dye. Fungal colorants were delivered in two metho...

Background: Current microscopic experimental methods cannot diagnose DNA damages present in spermatozoa .Therefore, some methods are needed to address the abnormality of the genetic material status on the sperm samples. As reported by many investigators aniline blue staining technique has been used for identifying sperm chromatin condensation. Also, chromomycin A3 is used for evaluation of the ...

in medicine, patent blue violet (pbv) is utilized for staining lymphatic vessels in sentinel lymph node (sln) surgery. moreover, pbv (also called e131 ) is used as food additive. we report on a 51-year-old non-atopic female with early breast cancer, who was scheduled for sln excision and experienced an intra-operative anaphylactic reaction. in diagnostics the skin prick test (spt) was positive ...

The ability of some bacteria and filamentous fungi to degrade aniline and its derivatives was reported earlier in the literature. However, there was no information about the biodegradation of aniline by yeast strains. The present work is focused on yeast strain Candida methanosorbosa BP-6 which was isolated from the wastewater pool of the old dye factory "Boruta" in Zgierz by enrichment techniq...

introduction: the novel development of the (hba) sperm- hyaluronan binding assay has now been routinely used in some laboratories worldwide to predict sperm maturity and functionality. the purpose of this study was to evaluate the correlation between hba score and histone deficiency, protamine content and dna fragmentation. methods: during april- march 2012 patients who were enrolled at shiraz-...

The present study focuses on predicting the concentration of intracellular storage polymers in enhanced biological phosphorus removal (EBPR) systems. For that purpose, quantitative image analysis techniques were developed for determining the intracellular concentrations of PHA (PHB and PHV) with Nile blue and glycogen with aniline blue staining. Partial least squares (PLS) were used to predict ...

Yeast hexokinase, a homodimer (100 kDa), is an important enzyme in the glycolytic pathway. Although Cibacron Blue 3G-A (Reactive Blue 2) has been previously shown to inactivate yeast hexokinase, no comprehensive study exists concerning the nature of interaction(s) between hexokinase and the blue dye. A comparison of the computer-generated three-dimensional (3D) representations showed considerab...

When uninjured cells of Valonia are placed in methylene blue dissolved in sea water it is found, after 1 to 3 hours, that at pH 5.5 practically no dye penetrates, while at pH 9.5 more enters the vacuole. As the cells become injured more dye enters at pH 5.5, as well as at pH 9.5. No dye in reduced form is found in the sap of uninjured cells exposed from 1 to 3 hours to methylene blue in sea wat...

We have come to divide bacteria and viruses into filterable and nonfilterable, and we have come to think that those organisms, visible or invisible, which are smaller than the pores of our filter are filterable. That size, however, cannot be the sole criterion we have known from the behavior of certain aniline dyes.* Thus Victoria blue, a basic dye, will not pass a Berkefeld filter while Congo ...