Metarhizium anisopliae is a fungus of considerable metabolic and ecological versatility, being a potent insect pathogen that can also colonize plant roots. The mechanistic details of these interactions are unresolved. We provide evidence that M. anisopliae adheres to insects and plants using two different proteins, MAD1 and MAD2, that are differentially induced in insect hemolymph and plant roo...

Bioassays were conducted to document the effects of Metarhizium anisopliae infection on adult female Asian longhorned beetle (Anoplophora glabripennis) reproduction before death and subsequent survival of offspring. The effect of infection on fecundity was evaluated for females already laying eggs and for newly eclosed females using M. anisopliae isolates ARSEF 7234 and 7711, respectively. Decr...

Ambient pH regulates the expression of virulence genes of Metarhizium anisopliae, but it was unknown if M. anisopliae can regulate ambient pH. Mutants of M. anisopliae altered in production of oxalic acid were evaluated for the interrelationship of ambient pH, buffering capacity added to media, growth, and generation of extracellular proteases and ammonia. Wild-type and acid-overproducing mutan...

Understanding mortality patterns and interactions between entomopathogenic fungi and parasitoids is important to improve insect biological control programs. The aim of this study was to evaluate the effect of Metarhizium anisopliae (Metschnikoff, 1879) Sorokin, 1833 and Beauveria bassiana (Balsamo) Vuillemin, 1912 (Hypocreales: Clavicipitaceae) on adults of Cotesia flavipes (Cameron, 1891) (Hym...

Repeated subculturing caused rapid changes in the spore surface properties and virulence of Metarhizium anisopliae. Of the two strains evaluated, M. anisopliae V245 attenuated more rapidly than V275. Electrophoretic mobility and Radial Flow Chamber assays were used for the first time to generate qualitative and quantitative information on the adhesive forces of M. anisopliae conidia. Independen...

Wholesale container-grown ornamentals are often maintained at the nursery for at least two growing seasons and are subject to infestation by black vine weevil (BVW), Otiorhynchus sulcatus F., for several months each year. Therefore, a potting media amendment aimed at controlling BVW needs to persist for an extended period of time. These studies were conducted to determine the persistence and ec...

Wedetermined that spinosad interacts synergistically with the biocontrol agentMetarhizium anisopliae (Metch) Sorokin to increase the mortality of two wild-collected wireworm species, Agriotes lineatus(L.), andAgriotes obscurus(L.).BioassayswereperformedusingaM.anisopliae isolate originally acquired from a local wireworm cadaver.M. anisopliaewas applied as a soil drench at 3.3 10 and 10 conidia ...

Ixodes scapularis Say adults, nymphs, and larvae were treated with the entomopathogenic fungus Metarhizium anisopliae in a combination of field and laboratory experiments to assess sublethal effects of the fungus on I. scapularis fecundity and body mass. Postengorgement and egg mass weights were 33 and 50% lower, respectively, in adult females treated with M. ansiopliae in the field before engo...