Evidence from animals suggests that anti-anthrax protective antigen (PA) immunoglobulin G (IgG) from vaccination with anthrax vaccine adsorbed (AVA) is protective against Bacillus anthracis infection. Measurement of anti-PA IgG in human sera can be performed using either enzyme-linked immunosorbent assay or fluorescent covalent microsphere immunoassay (ELISA) (R. E. Biagini, D. L. Sammons, J. P...

A branched beta-glucan from Sparassis crispa (SCG) is a major 6-branched 1,3-beta-D-glucan showing antitumor activity. In the present study, we examined the anti-SCG antibody in naive mice by ELISA. Using SCG coated plate, sera of naive DBA/1 and DBA/2 mice contained significantly higher titers of antibody than other strains of mice. Anti-SCG Ab titers of each DBA/1 and DBA/2 mice were signific...

Anti-La sera from patients with autoimmune disorders precipitate a set of nuclear and cytoplasmic small RNA-protein complexes. Up to now, it has been thought that the La antigen is associated only with RNAs transcribed by RNA polymerase 111, including precursors of tRNA and 5 S ribosomal RNA. Here we report that anti-La sera also react with ribonucleoprotein particles containing small nuclear ...

With eight of 32 sera significantly higher estimations of anti-D concentration were obtained when test cells premodified with bromelin were used instead of bromelin as a reagent added to the reaction manifold. With one serum the difference in estimation, using the two techniques, revealed a ten-fold discrepancy. The consequences of these findings are discussed in relation to the use of the Auto...

The complement system is a humoral effector in the innate immune system. Three activation pathways exist in the complement system, known as the classical pathway, the lectin pathway and the alternative pathway. Dysfunction of lectin pathway activation is caused by MBL deficiency. MBL deficiency in a cohort of healthy Caucasian blood bank donors was investigated with MBL genotyping and MBL plasm...

D adsorption and elution procedures are often helpful in separating multiple antibodies in a complex mixture. One of the applications of this procedure is to confirm the presence or absence of anti‐D, anti‐C, and anti‐G in the serum with apparent anti‐D + anti‐C specificities in alloimmunized pregnancies.[1] We performed a modified sequential differential adsorption‐ elution procedure in such p...

We have previously reported that, in addition to well-known pemphigus antigens (desmoglein and pemphigus vulgaris antigen), desmocollin (Dsc), another desmosomal cadherin, is recognized by certain pemphigus sera. Furthermore, we noticed that two Dsc species with a slightly different relative molecular weight were recognized by various pemphigus sera. This difference may contribute to the differ...

The present study comprised of 33'0 cases of infertility attending the outpatient section of the Department of Obstetries and Gynaecology, Jawaharlal Nehru Medical College, Aligarh. Only those cases were included where the semenograms of the male partners were normal. ABO blood grouping was done with high grouping sera and the final grouping was confirmed by matching the sera of the patients ag...

Dear Sir, G antigen (Rh12) is expressed on red blood cells that carry D and/or C antigen1-3. Serine is the critical amino acid for G antigen expression. Anti-G can cause haemolytic disease of newborn, which is rare, but responsible for neonatal morbidity and mortality. However, Anti-G antibody is difficult to distinguish from anti-D in combination with anti-C. Accurate identification of these a...

1. Antibodies to slime molds were produced by injecting D. discoideum and D. purpureum amebas from 48 hour cultures into rabbits. 2. Anti-D. discoideum and anti-D. purpureum sera caused agglutination of homologous amebas from 24 to 26 hour cultures, agglutination of certain heterologous amebas from 30 to 36 hour cultures, and agglutination of all heterologous amebas from 43 to 48 hour cultures....