Foci of recent cell death were identified in 10 consecutive cases of Huntington's disease in the caudate nucleus and putamen. Indirect immunohistochemical staining procedures were performed on these areas and controls using fluorescein conjugated anti-globulin and peroxidase-conjugated anti-globulin. Positive results were found when human antiserum to Cytomegalovirus was used. The possibility o...

background autoimmune hemolytic anemia is a hematologic disorder that is rarely observed in infants and young children. most of the cases are associated with viral or bacterial infections. in some cases, aiha can be characterized by a chronic course and an unsatisfactory control of hemolysis, thus requiring prolonged immunosuppressive therapy. case report especially in children younger than 2 y...

lsologous antigenic determinants of y-globulin revealed by proteolytic digestion of the globulin molecules were discovered by Osterland, Harboe, and Kunkel (1963) in human lgG and by Mandy, Fudenberg, and Lewis (1965) in rabbit y G. These determinants are detected by autologous and isologous antibodies found in most human sera and in all rabbit sera, respectively. These antibodies were named pe...

Through the use of absorbed idiotypic antisera prepared against single isolated monoclonal IgM anti-gamma-globulins, partial cross-idiotypic specificity was demonstrated with other IgM anti-gamma-globulins. Such antisera classified these proteins into at least three groups. The major group which included 60% of the anti-gamma-globulins was particularly homogeneous. The anti-gamma-globulin speci...

Infection of mice with the lactic dehydrogenase virus (LDV) resulted in an elevated level of gamma-globulin. Histologic examination of the spleen and lymph nodes revealed that the number of germinal centers was greatly increased. Immunization with human gamma-globulin showed that the capacity of the virus-infected animal to produce anti-human gamma-globulin was greatly enhanced and that the vir...

Rabbit antisera prepared against conjugates of the benzylpenicilloyl (BPO) bifunctional haptenic group were analyzed to determine whether the antibodies are adapted to only a portion of the large BPO molecule, or to the entire molecule, and whether specificity extends to the lysine side chain and adjoining structures of the immunizing carrier protein. No antibodies adapted to the phenylacetylam...

Human kidney mRNA species were isolated and fractionated through a continuous sucrose gradient ultracentrifugation. mRNA fractions were translated by using a rabbit reticulocyte lysate and [35S]methionine as tracer. Double immunoprecipitation was carried out with highly specific anti-human renin and anti-rabbit gamma-globulin antisera. A 15S mRNA has been found to direct synthesis of a 45 000 m...