B-cell antigen receptor (BCR) or antibody diversity arises from somatic recombination of immunoglobulin (Ig) gene segments and is concentrated within the Ig heavy (H) chain complementarity-determining region 3 (CDR-H3). We performed high-throughput sequencing of the expressed antibody heavy-chain repertoire from adult torafugu. We found that torafugu use between 70 and 82% of all possible V (va...

Underlying the development of antibody diversity is a unique pattern of gene organization and molecular events. THREE FAMILIES OF IMMUNOGLOBULIN GENES exist in mammals, one encoding HEAVY chains, another KAPPA chains, and the third LAMBDA chains. Each of these clusters contains one or more constant region genes and a number of variable region gene segments. The formation of a complete variable ...

The specificity of antibodies for different antigens appears to result from differences in the amino acid sequences of both the heavy and the light polypeptide chains of which antibody molecules are composed. -4 Although specific antibodies are too heterogeneous for complete amino acid sequence analysis, homogeneous iminunoglobulins may be obtained for this purpose from various plasma cell tumo...

The antibody response to Plasmodium falciparum parasites of naturally infected population is critical to elucidate the role of polymorphic alleles in malaria. Thus, we evaluated the impact of antigenic diversity of repetitive and family dimorphic domains of the merozoite surface protein 2 (MSP-2) on immune response of 96 individuals living in Peixoto de Azevedo (MT-Brazil), by ELISA using recom...

background: major histocompatibility complex (mhc) plays a central role in regulation and control of the immune responses to infectious diseases. due to its polymorphism, individual differences in response to vaccines have been observed in different chicken populations. studying the association of chicken mhc with immune response to vaccines will help the control of infectious disease and vacci...

In this paper, we argue that the metaphor form of a comparison (i.e., a topic-vehicle pair) is preferred over, and more comprehensible than, the simile form when the interpretive diversity for that comparison is high. Interpretive diversity refers to the richness of the figurative meaning of a comparison; it is high to the extent that more features or properties are related to the figurative me...

High-throughput antibody repertoire sequencing (Ig-seq) provides quantitative molecular information on humoral immunity. However, Ig-seq is compromised by biases and errors introduced during library preparation and sequencing. By using synthetic antibody spike-in genes, we determined that primer bias from multiplex polymerase chain reaction (PCR) library preparation resulted in antibody frequen...