background: previous studies revealed that selectins play key roles in homing of immune cells to inflamed tissues and lymphatic organs. l-selectins are expressed on immune cells and interact with p and e selectins to homing to the tissues, hence, the polymorphisms within the gene of l-selectins may are associated with alteration in its expression. thus, the current cross-sectional analytical st...

The reference method for immunoglobulin G (IgG) avidity determination includes reagent-consuming serum titration. Aiming at better IgG avidity diagnostics, we applied a logistic model for the reproduction of antibody titration curves. This method was tested with well-characterized serum panels for cytomegalovirus, Epstein-Barr virus, rubella virus, parvovirus B19, and Toxoplasma gondii. This ap...

The ELISA technique has been found to be reliable for the detection and titration of cytomegalovirus-specific IgG antibody in serum. It is about six times more sensitive than the CF test although some discrepancies were found between the antibody titres determined by the two methods.

Use of an automated apparatus to quantitate mumps virus- and poliovirus-neutralizing antibody is described. The automated titration equipment affords savings in effort, time, and reagents in conducting large-scale surveys for the determination of mumps- and poliovirus-neutralizing antibodies. This technique has been found to be reproducible and gives results comparable to other antibody assay m...

A simple and rapid method for the titration of virus-neutralizing antibody is described. The micro-apparatus and reagents are readily available from commercial sources. Results are included from selected cases which illustrate various conditions of immune status and response.

BACKGROUND Antibody titration is difficult to standardize. We investigated whether a detailed, uniform procedure for antibody titration would reduce variation in both tube-based and gel card titres in an international study. METHODS Laboratories (n = 35) tested proficiency testing material provided by the College of American Pathologists each according to (i) their routine method; (ii) a deta...