capture ELISA system was developed for diagnosis of visceral leishmaniasis (VL) using a monoclonal antibody raised against an antigen previously detected in the urine of VL patients. Urine samples from confirmed VL cases from Yemen, Nepal, Spain, Sudan and Brazil were tested in the capture ELISA in comparison with urine samples from endemic and non-endemic areas along with urine samples from pa...

towelve calves of 4-10 months old with clinical signs suspected to bovine viral diarrhea virus (bvdv) infection were selected for this study. histopathologic sections were performed on formalin fixed paraffin-embded ear notch biopsies, mounted on poly-l-lysine coated slides and stained for bvdv by anti-bvdv monoclonal antibody labelled with fluorescein isothiocyanate. stained sections were exam...

In this study a label-free electrochemical Immunosensor for ultrasensitive detection of Hepatitis C virus core antigen in serum samples was fabricated by using a simple approach. In this method a low-cost and sensitive immunosensor was fabricated based on a boehmite nanoparticles (BNPs) modified glassy carbon. The BNPs provide a specific platform with increased surface area which is capable of ...

Objective(s): Detection of circulating Mycobacterium tuberculosis (M. tuberculosis) antigens is promising in Tuberculosis (TB) diagnosis. However, not a single antigen marker has been found to be widely expressed in all TB patients. This study is aimed to prepare broadly reactive polyclonal antibodies targeting multiple antigen markers (multi-target antibodies) and evaluate their efficacies in ...

background: the aim of this study was to evaluate the possible use of biomphalaria alexandrina snail antigens in diagnosis of schistosomiasis mansoni using enzyme linked immunolectrotransfere blot (eitb). methods: s. mansoni adult worm crude antigens (awa), feet and visceral humps of b. alexandrina and bulinus truncatus were used. hyperimmune mice sera (his) versus each antigen were prepared fo...

Current work proposes an inimitable composite, with great electrical conductivity and quite enhanced surface area, (including conducting polymers (poly (cathechol)), Cu2O–carbon dots and green synthesized gold nanoparticles) for detecting acute carcinoembryonic antigen. At current work, the electropolymerization was offered instead of enzyme-catalyzed polymerization of poly (catechol). <b...

To develop an ELISA technique for the detection of antibodies against Babesia ovis, the infectederythrocytes were lysed and the supernatant soluble antigen, after sonication and ultracentrifugation of thelysate was used as antigen. Optimal dilution of the antigen was determined by checkerboard titrations, usingpositive and negative control sera. A correlation of 85% was observed between the res...

alkylhydroperoxide reductase (ahpc, the 26 kda antigen) is one of the abundant antioxidant enzymes in helicobacter pylori and seems to have a good potential for use in development of immunoassays to detect h. pylori infection in clinical specimens. this study aimed to investigate some properties of this antigen by the produced monoclonal antibodies. five established hybridoma cell lines secreti...

heartworm infection is one of the most important parasitic diseases in dogs and an increasing number of cases are reported each year by necropsy in ahvaz. this study was conducted to determine the prevalence of dirofilaria immitisinfection and to investigate the risk factors related to heartworm disease in urban dogs in this area. blood samples were collected from 100 dogs from feb 2007 to feb ...