Trichuris muris, the mouse whipworm, is used as a laboratory model of the human parasite T. trichiura. Three laboratory isolates of T. muris exist - the E, J and S isolates. Previous data have shown that the S isolate survives to chronicity in C57BL/6 mice unlike the E and J isolates, which are expelled. The ability of the S isolate to persist is thought to be due to it secreting unique excreto...

Immunophenotyping of acute leukemia using flow cytometry after density gradient separation (dg-sep) of mononuclear cells is the international gold standard. But destroying red cells by whole blood lysis (wb-lysis) after direct staining has found a broad usage. Both methods revealed congruent results in phenotyping of 26 cases of acute myeloid leukemias by testing CD4, CD7, CD11b, CD11c, CD13, C...

Obesity-associated inflammation is accompanied by the accumulation of adipose tissue macrophages (ATMs), which is believed to predispose obese individuals to insulin resistance. CD11b (integrin αM) is highly expressed on monocytes and macrophages and is critical for their migration and function. We found here that high-fat diet-induced insulin resistance was significantly reduced in CD11b-defic...

Overnight incubation of human eosinophils (Eøs) with the glucocorticoid hormone dexamethasone (DEX; 0.1 microM) resulted in lower expression of the CD11b, but not CD49d, antigen on their plasma membrane, as assessed by flow cytometry. DEX produced a consistent inhibitory effect (ranging from 16% to 20%) when tested at a concentration of 0.1 microM. Eø stimulation with 100 ng/ml eotaxin produced...

Conformational movement of the C-terminal alpha7 helix in the integrin inserted (I) domain, a major ligand-binding domain that adopts an alpha/beta Rossmann fold, has been proposed to allosterically regulate ligand-binding activity. Disulfide bonds were engineered here to reversibly lock the position of the alpha7 helix in one of two alternative conformations seen in crystal structures, termed ...

The antigenic phenotype of human synovial lining cells in normal and hyperplastic synovium intima was determined with a panel of monoclonal antibodies directed against a large number of well defined myeloid (macrophage/granulocyte associated) antigens. Synovial lining cells express numerous macrophage associated antigens, including CD11b (CR3), CD13, CD14, CD16 (FcRIII), CD18, CD32 (FcRII), CD4...

inflammation of blood vessels is a characteristic feature of kawasaki disease. neutrophils play a key role in the inflammatory responses where movement of neutrophils toward the site of inflammation depends on cd11b/cd18 expression as adhesion molecules on these cells. the purpose of this study was to investigate cd11b/cd18 expression in patients with kawasaki disease upon diagnosis and after t...

Background: It is still controversial that the stem cells isolated from human dental pulp meets the criteria for mesenchymal stem cells (MSCs). The aim of the present study was to examine whether or not they are MSCs, or are distinct stem cells population residing in tooth pulp. Methods: Adherent fibroblastic cells in the culture of pulp tissue from human third molars were propagated through se...

background: it is still controversial that the stem cells isolated from human dental pulp meets the criteria for mesenchymal stem cells (mscs). the aim of the present study was to examine whether or not they are mscs, or are distinct stem cells population residing in tooth pulp.   methods: adherent fibroblastic cells in the culture of pulp tissue from human third molars were propagated through ...

The immune system takes its cues from antigen-presenting cells (APCs) to either go on the offensive against a foreign enemy or to stand down and tolerate a harmless threat. Ehirchiou et al. (page 1519) now report a new mechanism by which APCs power down the immune system: an APC surface receptor suppresses the development of overzealous T helper (Th)-17 cells. According to dogma, APCs that have...