Long noncoding RNAs (lncRNAs) are increasingly recognized as involved in human physiology and disease. Antisense lncRNAs ubiquitous the genome have many mechanisms of action proposed, but single-cell single-molecule information that is needed to support or refute these lacking. Although single molecule RNA-Fluorescent Situ Hybridization (RNA-FISH) a method sometimes used investigate lncRNAs, co...

Antisense technology is based on the ability to design potent, sequence-specific inhibitors. The G-clamp heterocycle modification, a cytosine analog that clamps on to guanine by forming an additional hydrogen bond, was rationally designed to enhance oligonucleotide/RNA hybrid affinity. A single, context-dependent substitution of a G-clamp heterocycle into a 15-mer phosphorothioate oligodeoxynuc...

Abstract Using RNA antisense purification and mass spectrometry, we identified more than 100 human proteins that directly specifically bind SARS-CoV-2 in infected cells. To gain insights into the functions of selected interactors, applied genetic perturbation pharmacological inhibition experiments, mapped contact sites on viral RNA. This led to identification host dependency factors defense str...

Recent advances in molecular medicine have proposed new therapeutic strategies for cancer. One of the molecular research lines for the diagnosis and treatment of cancer is the use of long non-coding RNAs (LncRNAs) which are a class of non-coding RNA molecules longer than 200 base pairs in length that act as the key regulator of gene expression. Different aspects of cellular activities like cell...

The link between IL-13 and bronchial hyper-responsiveness has brought this cytokine as a potential therapeutic target for asthma and allergic diseases. At the present study, we address the role of B cell derived IL-13 in the IgE and other immunoglobulin development. Antisense oligo for human IL-13 m-RNA was used to study IgE down regulation. Human B-lymphocytes were purified by positive selecti...

Since its discovery 10 years ago, RNA interference (RNAi) has evolved from a research tool into a powerful method for altering the phenotype of cells and whole organisms. Its near universal applicability coupled with its pinpoint accuracy for suppressing target proteins has altered the landscape of many fields. While there is considerable intellectual investment in therapeutics, its potential e...

In this study, we employ antisense RNA technology to block Hepatitis B Virus (HBV) gene expression in cell culture by gene transfer as an approach to block immune recognition and pathogenic sequelae. Retroviral vectors encoding antisense and sense copies of the HBV surface antigen gene (HBsAg) were constructed, respectively. To assay the inhibition of HBV gene expression by antisense RNA, the a...

I n 1996 Worth magazine proclaimed that Isis Pharmaceuticals could become the next Microsoft, a prediction that turned out to be a particularly egregious example of hyperbole run amok. To be sure, Isis remains a leader in the gene-blocking technology called antisense. But the road to successful treatments for cancer and other diseases has been littered with disappointments. During the past few ...

Antisense oligonucleotides are potentially powerful tools for selective control of cellular and viral gene expression. Crucial to successful application of this approach is the specificity of the oligonucleotide for the chosen RNA target. Here we apply DNA array technology to examine the specificity of antisense oligonucleotide treatments. The molecules used in these studies consisted of phosph...

1477-3627/02/$ – see front matter ©2002 Elsevier Science Ltd.All rights reserved. PII: S1477-3627(01)02172-9 Sequencing of the human genome has provided the pharmaceutical industry with thousands of new potential drug targets, most of which operate intracellularly [1,2]. This fact opens new perspectives for therapy of human diseases but also demands new approaches in target validation. Differen...