introduction: among the endocrine diseases, hypothyroidism is the commonest after diabetes.thyroid hormones (t3,t4) are essential for genital organs function. apoptosis process in ovarian cells plays a significant role of development in ovarian follicles. the aim of the present study was determine the apoptotic index induced by hypothyroidism in rat ovarian follicles. materials and methods: in ...

objective: growth differentiation factor 9b (gdf9b) is an oocyte-derived growth factor. this protein is essential for the development of ovarian follicles and acts mainly by binding to its receptor on the surface of granulosa cells. the effect of gdf9b on the growth of follicles in various developmental stages, particularly primordial and primary follicles, is unknown. thus the aim of this stud...

The cellular localization of the inhibin subunits (α, β(A), and β (B)), steroidogenic enzymes (3β-hydroxysteroid dehydrogenase (3βHSD) and cytochrome P450 aromatase (P450arom) were evaluated in the ovaries of cyclic (n=6) and pregnant (n=2) Shiba goats (Capra Hircus). The immunointensity of inhibin α and β(A) subunits showed an increase in the granulosa cells (GC) of developing follicles. Inhib...

BACKGROUND Previous studies have demonstrated development of antral follicles in cryopreserved human ovarian tissue after autografting and xenografting, thus indicating successful preservation of follicular function. The study aim was to assess whether these follicles could also undergo periovulatory changes in response to hCG. METHODS Ovarian tissue from three patients were dehydrated in pro...

BACKGROUND Sono-automatic volume calculation (SonoAVC) automatically identifies and measures the dimensions of hypoechogenic areas within datasets acquired using three-dimensional ultrasound. The objective of this study was to evaluate the predictive value of automated antral follicle counts according to their relative sizes in women undergoing assisted reproduction treatment (ART). METHODS A...

Chick embryo extract (CEE) contains a variety of growth factors which may improve in vitro follicle growth. Therefore, the effect of CEE on mouse pre-antral follicle culture was evaluated. Different percentages of CEE (0, 0.50%, 1.00%, 5.00% and 10.00%) were added to culture medium. Hence, the osmolarity of media was measured. Pre-antral follicles with diameter of 120-150 μm were isola...

The pesticide methoxychlor (MXC) is a reproductive toxicant that targets antral follicles of the mammalian ovary. Cytochrome P450 enzymes metabolize MXC to mono-OH MXC (1,1,1-trichloro-2-(4-hydroxyphenyl)-2-(4-methoxyphenyl)ethane [mono-OH]) and bis-OH MXC (1,1,1-trichloro-2,2-bis(4-hydroxyphenyl)ethane [HPTE]), two compounds that are proposed to be more toxic than the parent compound, can inte...

The aim of this study was to evaluate the influence of two-dimensional (2D) and three-dimensional (3D) alginate culture systems on in vitro development of pre-antral caprine follicles. In addition, the influence of the reproductive age of the ovary donor on the in vitro culture success was investigated. Pre-antral follicles from pre-pubertal or adult goats were isolated and cultured directly on...

s:2022:"Background: Cryopreservation is an important option for preserving the fertility. We examined the reactive oxygen species (ROS) production, total antioxidant capacity (TAC) and developmental competence, of vitrified isolated mice pre-antral follicles as an alternative method for restoring fertility. Materials and Methods: The 140-160 μm pre-antral follicles mechanically isolated from ov...