PROBLEM Recurrent spontaneous abortion (RSA) is a common clinical problem with a complex etiology of genetic and non-genetic causes, which remains to be fully determined. IGF-2 stimulates trophoblast invasion, proliferation and maturation of placenta, while H19 RNA suppresses growth. As genomic imprinting plays a critical role in the development of placenta and embryo, our aim was to evaluate t...

Since listeriosis, caused by Listeria monocytogenes, is one of the important concerns of public health in Europe related to foodborne zoonoses, an efficient protocol for isolate typing is necessary when performing epidemiological studies. Three standardized PFGE protocols available for L. monocytogenes were briefly reviewed. Since observing a poor-quality of ApaI pulsotypes in our laboratory, e...

Background: Regular physical activity (PA) practice during pregnancy offers health and fitness benefits for both mother baby. Therefore, healthy pregnant women with no contraindications to exercise should be encouraged perform PA. Nevertheless, their levels of PA are generally low. The aim the WELL-DONE! Study is co-design an adapted intervention (APAI) include in childbirth preparation classes...

Methods There were examined 94 Ch aged 3-18 years with AD and duration of disease 1-15 years. Patients underwent the standard general clinical and immunological and allergy survey. The treatment consisted of 2 stages: traditional therapy (TT) for reducing disease complications, and IT. Patients were divided in 3 groups: I group (n=30) received stepped therapy with immune response-modulating age...

late-onset alzheimer's disease (ad), a genetically heterogeneous neurodegenerative disorder, is the most common form of dementia in people over 65 years old. the role of vitamin d in neuropsychiatric and neurodegenerative disorders such as ad has been supported by epidemiologic investigations and animal models, as well. we examined the association of the vitamin d receptor (vdr) gene polymorphi...

A physical and genetic map of Finegoldia magna (formerly Peptostreptococcus magnus) ATCC 29328 chromosome was constructed. The order of rare cleavage restriction fragments was determined by double digestion with the restriction enzymes I-CeuI, SgrAI, ApaI and PmeI, cross-hybridization and ApaI-linking clones. The size of the circular chromosome of F. magna was estimated to be 1.9 Mb. This strai...

Aim: This study aimed to investigate the correlation between rates of coronavirus disease 2019 (COVID-19) prevalence and mortality frequency vitamin D receptor (VDR) gene polymorphisms at loci rs7975232 (ApaI), rs1544410 (BsmI), rs2228570 (FokI), rs731236 (TaqI) in cohorts from 26 countries.Methods: The included use countries where VDR (TaqI), (BsmI) were determined relevant frequencies alleles...

BACKGROUND Polymorphisms of vitamin D receptor (VDR) contribute to the pathogenesis of multiple autoimmune conditions. METHODS We investigated the incidence of VDR polymorphisms (rs1544410-BsmI; rs7975232-ApaI; rs731236-TaqI) in a group of patients with primary sclerosing cholangitis (PSC, n = 275) and in healthy controls (n = 376). Additionally, correlations of the VDR polymorphisms with cli...