purpose: to ascertain whether the use of nested-pcr (nested polymerase chain reaction) technique leads to more rapid and accurate diagnosis of bacterial endophthalmitis. methods: the aqueous humor and vitreous samples of 24 eyes of 24 patients with bacterial endophthalmitis were evaluated by microscopy, diagnostic aerobic culture, and nested- pcr to detect the infectious agent. results: aerobic...

The anterior chamber of the eye is an immunologically privileged site. Over the past 15 years, numerous laboratories documented that the privileged status of this unique site is mediated by multifactorial immunoregulatory processes. Among the participating factors is the aqueous humor that circulates in the anterior chamber and is in contact with most of the tissues in the anterior segment of t...

PURPOSE To determine if a scrape injury to cat corneal endothelial cells increases the level of mitogenic proteins such as transforming growth factor alpha (TGF alpha) in aqueous humor. METHODS Aqueous humor of cats was collected at 0, 2, 6, and 24 hours after wounding the endothelium by contact with a cannula tip. Aqueous humor samples collected from sham-wounded cats served as controls. Aqu...

PURPOSE To determine whether aqueous humor promotes cell death in cells involved in inflammatory responses. METHODS Multiple immune cell types, most characteristically involved in inflammatory responses, were incubated for 24, 48, and 72 hours in the presence or absence of 50% aqueous humor. Promotion of cell death was assayed by staining for an early indicator of apoptosis. The percentage of...

HPLC/electrochemical detection was used to identify five major low MW water soluble electrochemically active molecules from the aqueous humor of three species of mammals: New Zealand White rabbits and humans (diurnal) and Sprague-Dawley rats (nocturnal). These molecules are L-cysteine (CYS), L-ascorbic acid (AA), glutathione (GSH), uric acid (UA) and L-tyrosine (TYR); all of these molecules hav...

OBJECTIVE To evaluate the plasma and aqueous humor disposition of prednisolone after oral administration in cats. METHODS Six cats were administered with a single oral dose of prednisolone (10 mg). Blood and aqueous humor samples were serially collected after drug administration. Prednisolone concentrations in plasma and aqueous humor were measured at 0.25, 0.5, 1.0, 1.5, 2.0, 3.0, 4.0, and 5...

Flow resistance was measured as bovine and primate aqueous humor was passed through Nuclepore polycarbonate filters having flow dimensions similar to those found within the juxtacanalicular meshwork of the aqueous outflow network. The results indicate that aqueous humor has a greater flow resistance than isotonic saline; this greater resistance is attributable to proteins or glycoproteins in aq...

IN a previous investigation a significant difference was found in the pressure response of the living and dead eyes of individual rabbits when a given volume of fluid was injected into the eye (Eisenlohr and Langham, 1962). An increase in the intra-ocular pressure of living animals was found to force blood from the eye and the amount lost per unit pressure increase was found to reach a maximum ...

.he ability of the cardiac glycosides, ouabain and digoxin, to lower eye pressure-' '' '-• has been ascribed to a primary inhibition of aqueous humor inflow. In the cat, which is the experimental animal of this study, the effect of ouabain on aqueous humor inflow is not clear. The previous report, which indicated that aqueous humor formation of the cat was decreased by ouabain, was based upon r...

PURPOSE To determine whether glaucoma patients exhibit an abnormal melatonin concentration in aqueous humor. DESIGN Case-controlled study, laboratory investigation. METHODS Aqueous humor and plasma samples of 28 patients with primary open-angle glaucoma and 31 nonglaucoma control patients were collected during surgery, and additional plasma samples were taken the night preceding surgery. Me...