Mitochondrial DNA (mtDNA) has been isolated from 4-cell stages of Ascaris lumbricoides. This DNA amounts to about 40 % of the total quantity of 4-cell-stage DNA. Its buoyant density in neutral CsCl gradients is 1 -686 g cm". Electron microscopy of mtDNA demonstrated the presence of circular molecules with an average contour length of 464 /<m. About 15 % of these molecules are supercoiled, coval...