The performances of two continuously monitoring mycobacterial culture systems-ESP Culture System II (ESP II; Trek Diagnostics, Inc. , Westlake, Ohio) and BACTEC MGIT 960 (BD Biosciences, Sparks, Md. )-were compared. In addition to both liquid media, all specimens were plated onto Middlebrook 7H11/7H11 selective agar. A total of 3, 151 specimens of all types (56.3% were respiratory specimens) we...

The ESP Culture System II was evaluated for its capacity to test the susceptibility of 389 cultures of Mycobacterium tuberculosis to streptomycin, rifampin, ethambutol, and isoniazid. Good agreement with results with the BACTEC TB 460 was found. ESP II is a reliable, rapid, and automated method for performing susceptibility testing.

introduction campylobacter jejuni is a slender, motile, non-spore-forming, helical-shaped, gram-negative bacterium. it is one of the most common causes of human gastroenteritis in the world. the aim of this study was to present a patient with acute lymphocytic leukemia (all), who was infected with campylobacter jejuni. conclusions c. jejuni should be considered in the differential diagnosis as ...

online | memorias.ioc.fiocruz.br Early detection of drug resistance in Mycobacterium tuberculosis isolates allows for appropriate and timely treatment (Martin et al. 2008). There are many welldefined phenotypic methods for testing the drug susceptibility of M. tuberculosis. The proportion method, performed on Löwenstein-Jensen and 7H10 or 7H11 agar media, is recommended as a reference method. H...

BACKGROUND For the diagnosis of prosthetic joint infection (PJI) automated BACTEC™ blood culture bottle methods have comparable sensitivity, specificity and a shorter time to positivity than traditional cooked meat enrichment broth methods. We evaluate the culture incubation period required to maximise sensitivity and specificity of microbiological diagnosis, and the ability of BACTEC™ to detec...

SETTING Mycobacteria growth in media with the addition of inhibitory substances has been used in species identification. Growth of the Mycobacterium tuberculosis complex (MTC) is inhibited by rho-nitrobenzoic acid (PNB), whereas non-tuberculous mycobacteria (NTM) are resistant. OBJECTIVE To develop a rapid PNB test using the automated BACTEC MGIT960 system and to evaluate its usefulness in th...

BACKGROUND Mycobacterium species are grown using specific media that increase laboratory cost, thus hampering their diffusion in resource-limited countries. Preliminary data suggested that versatile blood agar may be also used for mycobacterial culture. METHODOLOGY We examined the growth of 41 different Mycobacterium species on 5% blood agar. Over a 24-month period we analysed isolation of my...