The phytostimulatory properties of Azospirillum inoculants, which entail production of the phytohormone indole-3-acetic acid (IAA), can be enhanced by genetic means. However, it is not known whether this could affect their interactions with indigenous soil microbes. Here, wheat seeds were inoculated with the wild-type strain Azospirillum brasilense Sp245 or one of three genetically modified (GM...

Procedures based on DNA hybridization and PCR were developed for quality control of Rhizobium inoculants. Inoculants for pea and goat's rue were produced by Elomestari Ltd., Juva, Finland, in sterile dry fine peat by the standard procedure used by the company. The inoculants contained Rhizobium galegae HAMBI 1174 and HAMBI 1207 and an R. leguminosarum biovar vicia strain, 16HSA, either solely o...

The present study deals with the isolation of plant growth promoting rhizobacteria (PGPR) from rice (variety NIAB IRRI-9) and the beneficial effects of these inoculants on two Basmati rice varieties. Nitrogen-fixing activity (acetylene-reduction activity) was detected in the roots and submerged shoots of field-grown rice variety NIAB IRRI-9. Estimation of the population size of diazotrophic bac...

The ecological examination of members of the family Rhizobiaceae has been hampered by the lack of a selective medium for isolation of root nodule bacteria from soil. A novel non-antibiotic-containing medium has been developed which allows selective isolation of Bradyrhizobium japonicum and B. elkanii strains from soil and inoculants. The medium, BJSM, is based on the resistance of B.japonicum a...

Nothing is more important for effective nodulation of legumes that the availability of sufficient numbers of an infective strain of rhizobia in the root zone of seedlings. In some cases, the soil will contain sufficient numbers of infective rhizobia but modern approaches to the use of genetically superior strains of rhizobia practically demands the use of some means of inoculation, thus raising...

The restoration of submerged macrophytes is commonly limited by abiotic stress. Here, we isolated PGPR strains from the rhizosphere using ACC deaminase selective medium, and evaluated their growth promoting effects on Vallisneria natans (V. natans) under low light intensity (or) high sediment organic matter load, also explored indigenous microbiome response V. seedlings to inoculants. Ten isola...

A pot experiment was carried out during 2009 and 2010 to study the field performance of blue pine seedlings inoculated with selected species of bio-inoculants under nursery conditions .The experiment was laid in Completely Randomized Design with three replications which comprised of seven inoculants (Azotobacter sp., Azospirillum sp., Pseudomonas fluorescens, Bacillus subtilis, Pisolithus tinct...