Horizontal DNA transfer contributes significantly to the dissemination of antibiotic resistance genes in Bacteroides fragilis. To further our understanding of DNA transfer in B. fragilis, we isolated and characterized a new transfer factor, cLV25. cLV25 was isolated from B. fragilis LV25 by its capture on the nonmobilizable Escherichia coli-Bacteroides shuttle vector pGAT400DeltaBglII. Similar ...

A selective medium was developed for the isolation of Bacteroides fragilis directly from ovine and bovine fecal samples. The medium (tryptose blood agar plus polymyxin B, triclosan (Irgasan), novobiocin, and nalidixic acid) permitted growth of B. fragilis and several other species of Bacteroides but inhibited growth of most other intestinal bacteria.

conclusions the presence of bft gene in b. fragilis isolates wouldnot certainly result in diarrheaamong patients. there is not enough accordance between ddm and swedish e tests for antimicrobial susceptibility of b. fragilis for some antibiotics, although in other cases a good agreement was observed. background enterotoxigenic bacteroides fragilis (etbf) are one of the most important anaerobic ...

Two multidrug-resistant Bacteroides fragilis clinical isolates contain and express a novel nim gene, nimJ, that is not recognized by the "universal" nim primers and can confer increased resistance to metronidazole when introduced into a susceptible strain on a multicopy plasmid. HMW615, an appendiceal isolate, contains at least two copies of nimJ on its genome, while HMW616, an isolate from a p...

Bacteroides spp. has gained substantial interest among the suggested potential candidates for alternative faecal indicators for untreated recreational waters by the US EPA. Interest in Bacteroides as a faecal indicator is based upon the relative abundance of selected members of the Bacteroides genus in the human colon and human faeces. In this study, we developed a real-time PCR detection syste...

In this study, we show that Bacteroides species utilize Fe(III)-xenosiderophores as the only source of exogenous iron to support growth under iron-limiting conditions in vitro anaerobically. Bacteroides fragilis was the only species able to utilize Fe(III)-ferrichrome while Bacteroides vulgatus ATCC 8482 and Bacteroides thetaiotaomicron VPI 5482 were able to utilize both Fe(III)-enterobactin an...

The activity of cefotetan against 430 strains of anaerobic bacteria was compared with that of cefoxitin, ceftizoxime, clindamycin, metronidazole, and chloramphenicol. Percent susceptible values for the Bacteroides fragilis group were 60, 80, 29, 86, 100, and 100%, respectively. Percent susceptible values for the B. fragilis species were 91, 92, 46, 98, 100, and 100%, respectively. Non-B. fragil...

background diarrhea is the most frequent health problem among children in developing countries. defining the etiology of acute diarrhea is critical to disease therapy and prevention. some anaerobic bacteria such as enterotoxigenic bacteroides fragilis (etbf) strains cause diarrheal disease by production of enterotoxin in children less than 5 years old. objectives this study aimed to evaluate th...

Adding 0.05% cysteine to brain-heart infusion broth greatly improved the recovery of three strains of Bacteroides fragilis from simulated blood cultures. No bacteroides were isolated after 24 hours' incubation in brain-heart infusion broth without added cysteine, and this was therefore a poor medium for the isolation of B. fragilis. Results with brain-heart infusion broth containing 0.05% cyste...