903 a different perception on rhinitis, be it caused by an IgEor a non -IgE -mediated challenge, because CD63 is easily activated by nonallergic stimulation.4 Furthermore, the ability to perform an accurate BAT depends also on the gating strategy used by the test to catch basophils in the flow cytometry electronic capture.5 The Flow2CASTTM (BÜHLMANN, Schönenbuch, Switzerland) used by Leśniak et...

AIM To evaluate markers of mast cell and basophil activation in children undergoing the initial phase of honeybee venom immunotherapy (VIT). PATIENTS & METHODS Five children (four boys and one girl) aged 9.5-18 years with severe systemic bee sting reactions and confirmed IgE-mediated allergy were enrolled. Plasma and urine concentrations of 9α,11β-PGF2 and serum tryptase levels were measured ...

BACKGROUND Basophil activation test (BAT) reproduces IgE-mediated allergic reactions in vitro and has been used as a diagnostic test. Different markers can be used to identify basophils in whole blood and have implications for the outcome of the test. We aimed to assess changes in the expression of CD123 and HLA-DR following basophil activation and to select the best gating strategy for BAT usi...