Pyridoxal dehydrogenase is specific for DPNf and does not oxidize benzaldehyde, acetaldehyde, salicylaldehyde, or Sdeoxypyridoxal. In the presence of the latter compound, oxidation of pyridoxal is stimulated, apparently because of the occurrence of the dismutation reaction (ii). Z-Demethyl-Z-ethylpyridoxal is dehydrogenated at the same rate as pyridoxal. At high substrate concentrations the pH

Biochemical and histochemical studies were conducted in aflatoxin B1-induced liver tumors in adult rainbow trout. Specific activities of the phase I enzymes, ethoxyresorufin-O-deethylase (EROD), microsomal and cytosolic epoxide hydrolase (mEH and cEH), aldehyde dehydrogenase (ALDH) and DT-diaphorase, and the phase II enzymes, gamma-glutamyltransferase (gamma-GT), glutathione transferase (GST) a...

BACKGROUND Benzaldehyde dehydrogenase (BZDH) is encoded by the xylC that catalyzes the conversion of benzaldehyde into benzoate in many pathways such as toluene degradation. OBJECTIVES In this study, the xylC gene from Rhodococcus ruber UKMP-5M was expressed in Escherichia coli, purified, and characterized. MATERIALS AND METHODS The xylC was amplified and cloned in E. coli. The recombinant ...

The title compound, C(12)H(10)N(4)O, is a Schiff base obtained from the condensation of diamino-maleonitrile and 2-hy-droxy-3-methyl-benzaldehyde. The mol-ecule is roughly planar, with an r.m.s. deviation of 0.0354 Å, and adopts the phenol-imine tautomeric form. An intra-molecular O-H⋯N hydrogen bond involving the O-H group and the azomethine N atom generates an S(6) ring. In the crystal, there...

The enzymes of the mandelate metabolic pathway permit Pseudomonas putida ATCC 12633 to utilize either or both enantiomers of mandelate as the sole carbon source. The genes encoding the mandelate pathway were found to lie on a single 10.5-kb restriction fragment. Part of that fragment was shown to contain the genes coding for mandelate racemase, mandelate dehydrogenase, and benzoylformate decarb...

Aryl metabolite biosynthesis was studied in the white rot fungus Bjerkandera adusta cultivated in a liquid medium supplemented with L-phenylalanine. Aromatic compounds were analyzed by gas chromatography-mass spectrometry following addition of labelled precursors ((14)C- and (13)C-labelled L-phenylalanine), which did not interfere with fungal metabolism. The major aromatic compounds identified ...

Due to the drawbacks of applying catalysts in the synthesis of α,β-unsaturated structure units and the importance of these materials, electrochemistry has been introduced as an efficient alternative.Therefore, herein a high-yield synthesis of 2-arylideneindan-1,3-diones is proposed. The procedure is carried out in propanol, using electrons as a green catalyst for generating propanol anion...

HEN extracts of wild-type Drosophila are electrophoresed on agar gels and the gels stained with a tetrazolium solution containing benzaldehyde, a formazan band is formed at a location not identical to the xanthine dehydrogenase (XDH) band ( COURTRIGHT 1966a). This was a surprising observation since benzaldehyde has been reported to serve as a substrate for XDH (GLASSMAN and MITCHELL 1959a). Sin...