Lutropin (LH) bears asparagine-linked oligosaccharides terminating with the unique sequence SO4-4GalNAc beta 1-4GlcNAc beta 1-2Man alpha, whereas follitropin (FSH) bears oligosaccharides terminating predominantly with the sequence Sia alpha-Gal beta 1-4GlcNAc beta 1-2Man alpha, where Sia is sialic acid. We previously identified a glycoprotein-hormone-specific N-acetylgalactosamine-transferase (...

INTRODUCTION We describe a case of non-small cell lung cancer that was found to stain positive for beta-human chorionic gonadotropin on immunohistochemistry. Only a few case reports have described lung cancers that secrete beta-human chorionic gonadotropin. CASE PRESENTATION A 68-year-old Caucasian man presented with symptoms of weakness, fatigue and weight loss for the past two months. On ex...

Three clones of ectopic hormone-producing cells from a single human neoplasm (bronchogenic carcinoma) show different rates of synthesis and secretion of chorionic gonadotropin and its alpha and beta subunits. For each clone, the amount of one or the other subunit always exceeded that of the complete hormone molecule. These findings may be analogous to unbalanced immunoglobulin chain synthesis i...

The malignant trophoblastic cell line JAR was used as a model system to study protein folding in intact cells. We have used this model previously to identify conformational intermediates in the production of an assembly-competent form of the human chorionic gonadotropin beta subunit (Ruddon, R. W., Krzesicki, R. F., Norton, S. E., Beebe, J. S., Peters, B. P., and Perini, F. (1987) J. Biol. Chem...

the purification of biomolecules is a necessary step in many biochemical researches. in this regard, developments of convenient, specific and low cost methods of purification are of particular interest. given the human hemoglobin (hb) affinity toward some charged carbohydrates, interaction of this molecule with human chorionic gonadotropin (hcg) which is a glycoprotein hormone containing sialic...

The beta subunits of equine lutropin and equine chorionic gonadotropin were incubated in 0.013 N HCl for 30 min at 110 degrees C and separated into two fragments by reverse-phase high performance liquid chromatography. The amino acid and carbohydrate compositions of both fragments from each subunit were analyzed. The results demonstrated that equine lutropin-beta has a glycosylated COOH-termina...

Within the last year, human chorionic gonadotropin (hCG) has been a topic of great discussion in both the scientific and popular press. Recent reports have demonstrated that because of hCG’s heterogeneity, assay standardization is an important problem, with different immunoassays giving different results for the same specimens. In addition there have been reports of falsenegative results due to...