We report here that certain drugs can protect Syrian baby hamster kidney cells (BHK) in culture against the lethal agents cytosine arabinonucleoside, hydroxyurea, and colcemid. Polyoma virus-transformed BHK cells (PyBHK) are killed under the same conditions. The protective drugs include caffeine and streptovitacin A. Kinetic studies show that these drugs act specifically in G1, and that they sh...

MM virus (mouse-brain stock) replicated to a limited extent in L cells without cytopathic effects; the average yield was less than 1 plaque-forming unit/cell. Passage in BHK-21 cells resulted in MM virus [MM(BHK)] which replicated to high titers (200 to 300 plaque-forming units/cell) in L cells with complete cytopathic effects. Appearance of mature MM(BHK) virus in L-cell cultures begins 4 hr a...

The translational requirement to prevent premature termination during La Crosse virus S mRNA synthesis was found to be cell-type dependent. This requirement was present in the BHK, HEL, and Vero cell lines we examined, but not in C6/36 mosquito cells. The cell-dependent translational requirement could be reproduced in vitro by using either cell extracts or purified virions of BHK and C6/36 cell...

Previous studies suggest that the baby hamster kidney (BHK) cell fibronectin receptor is also a wheat germ agglutinin receptor (WGA-R). To analyze this possibility further, IgG and Fab fragments of antibodies produced against a BHK cell WGA-R preparation were tested to determine their effects on cell adhesion mediated by fibronectin, wheat germ agglutinin, concanavalin A, and polycationic ferri...

Chinese hamster ovary (CHO) cells are traditionally regarded as nonpermissive cells for herpes simplex virus type 1 (HSV-1) infection as they lack the specific entry receptors, and modified CHO cells have been instrumental in the identification of HSV-1 receptors in numerous studies. In this report we demonstrate that the HSV-1 strain 17+ variant HSV1716 is able to infect unmodified CHO cells b...

Normal and transformed baby hamster kidney (BHK) cells attach to Falcon polystyrene with the same first order rate constant. The longer the cells are attached to the bottles, the more difficult they are to remove. Sulfhydryl (-SH) binding reagents inhibit both the attachment of BHK cells and the increase in adhesive strength of attached cells. Attached BHK cells bind fewer molecules of [1-(14)C...

A tissue culture (TC)-fluorescent antibody (FA) technique for the measurement of rabies-neutralizing antibody was found to be reliable and comparable to the standard mouse serum neutralization test. This test was performed with BHK-21 cells infected with the ERA vaccine virus strain on Lab-Tek TC chamber slides. A Flury high egg passage (HEP) rabies virus strain grown on continuous line of Afri...

For establishment of plaque assay on BHK-21(Cl-13) cell line, different parameters were standardized. A cell count of 3-4 X 10 5 /ml was found suitable when grown in Eagle’s minimum essential medium (EMEM) containing 5% foetal bovine serum (FBS) whereas a concentration of 2% FBS was optimum for the overlay media containing 0.2% final conc. of carboxymethyl cellulose (CMC); a pH value of 7. 8 wa...

Scavenger receptor class B type I (SR-BI) mediates the selective uptake of HDL cholesteryl esters (CEs) by the liver. Hepatic lipase (HL) promotes this lipid uptake independent from lipolysis. The role of SR-BI in this HL-mediated increase in selective CE uptake was explored. Baby hamster kidney (BHK) cells were transfected with the SR-BI cDNA yielding cells with SR-BI expression, whereas no SR...

Thionins, which are high-sulphur polypeptides present in the endosperm of wheat and related species, have ben found to prevent growth and to inhibit macromolecular synthesis in cultured mammalian cells. Baby hamster kidney (BHK) cells were markedly more sensitive to thionins than the other cell lines tested (monkey CV1, mouse L, human HeLa). A thionin concentration of 5 microgram/ml (1 microM) ...