Objective: Teriflunomide is used for the treatment of multiple sclerosis and available in 7mg 14mg tablets. This study aimed to develop validatea simple economical HPTLC method estimation human plasma. Methods: was developed using toluene: ethyl acetate: acetic acid as mobile phase stationary a TLC plate precoated with silica gel 60 F254. The detection wavelength set 294nm. sample preparation i...

Foods are complex mixtures of lipids, carbohydrates, proteins, vitamins, organic compounds, and other naturally occurring substances. Sometimes added to this mixture are residues of pesticides, veterinary and human drugs, microbial toxins, preservatives, contaminants from food processing and packaging, and other residues. This milieu of compounds can pose difficulties in the analysis of food co...

www.pharmtech.com evelopment and validation of bioanalytical methods for pharmaceutical product analysis are common rate-limiting steps in biopharmaceutical product development. A rational, strategic approach to developing robust, automated, validated bioanalytical methods can reduce slowdowns and bottlenecks in drug development and contribute to synergistic, consistent, longterm performance. T...

In USA and Europe, medicines agencies force the development of child-appropriate medications and intend to increase the availability of information on the pediatric use. This asks for bioanalytical methods which are able to deal with small sample volumes as the trial-related blood lost is very restricted in children. Broadly used HPLC-MS/MS, being able to cope with small volumes, is susceptible...

* corresponding author. e-mail: [email protected] A bioanalytical protocol based on the enzymatic-spectrophotometric micro-method for determination of citrate with immobilized citrate lyase (CL) is described. In the design of this spectrophotometric bioanalytical system, L-malate dehydrogenase (L-MDH), oxaloacetate decarboxylase (OACD) and L-lactate dehydrogenase (L-LDH) were applied as a biologica...

Bioanalytical methods are widely used for quantitative estimation of drugs and their metabolites in physiological matrices. These methods could be applied to studies in areas of human clinical pharmacology and toxicology. The major bioanalytical services are method development, method validation and sample analysis (method application). Various methods such as GC, LC-MS/MS, HPLC, HPTLC, micella...

A rapid, specific, and reliable LC-MS/MS-based bioanalytical method was developed and validated in rat plasma for the simultaneous quantitation of amitriptyline and its metabolite nortriptyline. Chromatographic separation of these analytes was achieved on a Gemini C18 column (50 × 4.60 mm, 5 μm) using reversed-phase chromatography. The mobile phase was an isocratic solvent system consisting of ...