A new bioassay has been developed combining the simplicity of direct bioautography with the improved chromatographic resolution of 2D-TLC. Mixtures of structurally diverse antifungal agents were tested to establish the validity and utility of this method in the discovery of new natural products with activity against agriculturally important fungal pathogens.

The use of high-voltage electrophoresis and bioautography for the separation mixtures of antibiotics received in clinical serum samples is described. The electrophoretic characteristics of 39 antibiotics are given as is the accuracy of the method for quantitating three antibiotics. In screening 189 consecutive serum samples submitted for routine antibiotic assay, undisclosed antibiotics were r...

A microbioautographic method for the identification of pteroylglutamic acid derivatives and their glutamyl conjugates is described. Chromatograms or pherograms of mixtures of the compounds are treated with chicken pancreas conjugase, followed by tetrazolium bioautography. This combined procedure allows qualitative and semi-quantitative analyses of folate compounds in biological material in the ...

Objective: The objective of the study was to carry out phytochemical profiling essential oils (EOs) and evaluation their anti-microbial activity. Methods: EOs extracted from Cumminum cyminum, Zingiber officinale, Trachyspermum ammi, Alipnia galanga, Cedrus deodara, Elettaria cardamomum using clavenger apparatus. Phytochemical analysis high-performance thin layer chromatography (HPTLC) fingerpri...

As a widely used food preservative, methyl paraben was experimentally evidenced with serious hormone-like adverse effect. Herein, high performance thin-layer chromatography platformed bioluminescent bioautography and image analysis for the selective quantification confirmation of proposed validated in vinegar coconut juice. First, detectability to analyte on different layer materials estimated,...

The increasing occumnce of opportunistic systemic mycoses associated with the use of immunosuppressive drugs and AIDS has led to new efforts in the search for novel antifungal compounds. At the same time, there is continuing interest in the discovery of antifungal agents which are effective against plant pathogenic fungi. Since the plant kingdom provides a useful source of lead compounds of nov...

the present work was designed to study the antioxidant activity and to identify the main active components of the essential oil of ajowan (trachyspermum copticum) fruit. gc and gc-ms analyses of the essential oil showed the presence of eight compounds. the main constituents of the oil were thymol (43.7%), p-cymene (26.8%), and γ-terpinene (24.9%). the antioxidant and free radical scavenging act...