Induced mutagenesis causes an increase variation of some products with limited genetic resources. In this study, the antioxidant enzymes activity, biochemical properties and glycoside content of Stevia were assessed by EMS (chemical mutagen) based on two experiments as factorial with completely randomized design with three replications. The results of the first experiment showed that some prope...

How can a new incoming biological node measure the degree of nodes already present in a network and thus decide, on the basis of this counting, to preferentially connect with the more connected ones? Although such explicit comparison and choice is quite plausible in the case of man-made networks, like Internet, leading the network to a scalefree topology, it is much harder to conceive for bioch...

The electrophoretic pattern (in polyacrylamide gel) for acid phosphatases in the prostate gland was compared with that for other tissues. lsoenzyme 2 predominates in the prostate. The isoenzyme was isolated from the prostate and its biochemical properties were compared with those of acid phosphatases isolated from spleen. lsoenzyme 2 has a molecular weight of about 100,000. Its optimum pH is be...

In this experiment the effects of lead and lead-free petrol applied at the following doses of: 0, 2, 4 and 6 cm • kg' of soil on growth and development of triticale and biochemical properties of the soil were studied. For detoxication of petrol organic amendment with barley straw was applied. The experiment was performed in two experimental series with and without triticale cover. It was found ...

The biochemical properties of food allergens exert a large influence on how these allergens act when ingested, and determines important aspects such as cross-reactivity, lability to degradation during digestion or stability during processing of the food. One of the targets for biochemical characterization of food allergens is to establish a link between the biochemical data and the biological a...

Laccases (benzenediol: oxygen oxidoreductase, EC 1.10.3.2) are multicopper oxidases that are widely distributed among plants, insects, and fungi. They belong to a group of polyphenol oxidases containing copper atoms in the catalytic centre which are usually called multicopper oxidases. It utilizes molecular oxygen to oxidize various aromatic and non-aromatic compounds. They have been described ...

Increasing incidences of diabetes in Africa has prompted the search for safe and readily available alternative herbal remedies for the treatment of diabetes mellitus. Cnestis ferruginea was extracted with methanol and ethylacetate and the extracts obtained were tested for hypoglycaemic activities in streptozotocin (STZ)-induced diabetic rats and mice. The extracts (250 mg/kg body weight) were a...

A fragment of chromosomal DNA encoding the lgtE gene of Neisseria gonorrhoeae strain F62 was amplified by PCR and cloned into the expression vector pET15b. Functional LgtE was purified and its biochemical properties were determined. The purified enzyme was maximally active in buffer containing manganese; minimal activity was obtained in buffer containing other divalent cations. LgtE was only ab...

Lactate dehydrogenase (LDH, E.C.1.1.1.27) was found in supernatant (cytoplasmic enzyme) fractions of the trypanosomatid flagellates Trypanosoma conorhini and Crithidia fasciculata if 10 mm cysteine was present in the homogenizing medium. The T. conorhini LDH activity with pyruvate as substrate was increased 35% if 5 mm cysteine was also included in reaction mixtures. K(m) values for the T. cono...