Structural preservation of complex biological systems from the subcellular to whole organism level in robust forms, enabling dissection and imaging while preserving 3D context, represents an enduring grand challenge in biology. Here we report a simple immersion method for structurally preserving intact organisms via conformal stabilization within silica. This self-limiting process, which we ref...

Fixation is usually the first step to prepare biological specimens for microscopy. Any treatment which will preserve cell structure and its biochemical composition can be deemed to be fixation. Of course, the quality of fixation is the key for all following steps which are necessary in histological research. Hence, preservation of cells with minimal alteration of morphology and virtually no los...

Gender and sex diverse individuals-transgender individuals and those with disorders of sex development (DSD)-both face medical treatments that may impair biological fertility potential. Young DSD patients also often have abnormal gonadal development. Fertility preservation for these populations has historically been poorly understood and rarely addressed. Future fertility should be discussed wi...

^ In most laboratories in the North American Continent and in England, the older methods of Maintaining bacterial cultures by constant subculturing in suitable media have of recent years been largely superseded by the method of rapid freezing and dehydration in vacuo. As early as 1896, Martin suggested the possibility and Procedure involved in the preservation of guinea-pig complement in the dr...

Introduction: The response of organic matter to impact events is of widespread interest for what it tells us about the likelihood of survival of life and/or fossilized biological signatures [1,2,3] following impacts. This is relevant to the history of life on Earth, and to our exploration of other solar system bodies, including Mars. The Tertiary Haughton Impact Structure, Cana-dian High Arctic...

Following tooth extraction, the alveolar ridge undergoes an inevitable remodeling process that influences implant therapy of the edentulous area. Socket grafting is a commonly adopted therapy for the preservation of alveolar bone structures in combination or not with immediate implant placement although the biological bases lying behind this treatment modality are not fully understood and often...

Since the advent of cryo-electron microscopy (cryo-EM), the close-to-life and fine structure preservation of biological samples has become a realized procurement, surpassing the fixation quality and avoiding the numerous artifacts inherent to conventional chemical preservation methods. Cryo-EM techniques such as high-pressure freezing followed by freezesubstitution (HPF/FS) have facilitated the...

Gene coexpression network modules provide a framework for identifying shared biological functions. Analysis of topological preservation of modules across datasets is important for assessing reproducibility, and can reveal common function between tissues, cell types, and species. Although module preservation statistics have been developed, heuristics have been required for significance testing. ...