Introduction: Extracellular vesicles (EVs) are bacterial products with diverse biological roles. Like many microorganisms, Mycobacterium kansasii as a nontuberculous mycobacteria (NTM), can naturally release EVs. The aim of the present study was the extraction and biological evaluation of M. kansasii as a vaccine candidate against mycobacterial pulmonary infections. Methods: After bacterial cul...