BACKGROUND Acinetobacter baumannii is a significant hospital pathogen, possessing a considerable degree of antimicrobial resistance. A. baumannii resistance to carbapenems and aminoglycosides is mostly conferred by class D OXA carbapenemases and aminoglycoside-modifying enzymes, respectively. The aim of this study was to determine the prevalence of selected genes encoding OXA carbapenemases and...

Rapid molecular identification of carbapenemase genes in Gram-negative bacteria is crucial for infection control and prevention, surveillance and for epidemiological purposes. Furthermore, it may have a significant impact upon determining the appropriate initial treatment and greatly benefit for critically ill patients. A novel oligonucleotide microarray-based assay was developed to simultaneou...

Background: The emergence of multidrug-resistant Acinetobacter baumannii complicates the therapy of the related infections. Hospital isolates of A. baumannii are usually multidrug-resistant. The problem is compounded by increasing resistance to broad-spectrum antibiotics including carbapenems. Objectives: The aim of this study was to determine antimicrobial susceptibility patterns and distribut...

We distinguished the four OXA-type carbapenemase subgroup alleles present in 120 strains of Acinetobacter baumannii by using polymerase chain reaction (PCR) and investigated the distributions of the OXA subgroups in clinically isolated samples. Amplification of the OXA genes blaOXA-23, blaOXA-24, blaOXA-51, and blaOXA-58 was performed by multiplex PCR. Antibiotics susceptibility test was conduc...

Objectives: This study aimed to determine the Enterobacteriaceae strains among patients who suffering diarrhea, phenotypic and genotypic characterization of ESBL-producing isolates in Jeddah, Saudi Arabia.
 Methods: Stool samples were collected cultured different strains, PCR was done for detect ESBLs genes antibiotic susceptibility against antibiotics done.
 Results: The total number...

We aimed to investigate the clonal relationships, common sequence types, and carbapenemase genes in 177 non-repetitive blood culture isolates of Acinetobacter baumannii collected from patients at three university hospitals Turkey 2016. Molecular epidemiological characteristics were examined using pulsed-field gel electrophoresis (PFGE), multilocus typing (MLST) (Pasteur scheme-cpn60, fusA, gltA...

BACKGROUND/AIM The aim of this study was to determine the molecular characteristics and antibiotic resistance of 13 (10 inpatient and three outpatient) Acinetobacter baumannii beta-lactamase-producing isolates collected in Bosnia and Herzegovina between December 2009 and May 2010. MATERIALS AND METHODS Susceptibility testing was performed by disk diffusion and broth microdilution methods. The...

Emergence of Acinetobacter baumannii with resistance or reduced susceptibility to carbapenems has been reported worldwide (4). Acinetobacter may develop resistance to carbapenems through various mechanisms, including decreased permeability, efflux pump overexpression, and production of carbapenemases (3, 4, 9). Four of the eight carbapenemase-hydrolyzing oxacillinase (OXA) clusters have been id...

An IMP-4-producing Acinetobacter pittii strain coproducing oxacillinases was isolated from a leg wound of a 67-year-old female patient. Identification to the species level by rpoB and gyrB sequencing and multiplex-PCR-based analysis revealed that the isolate was A. pittii. Whole-genome sequencing of this A. pittii isolate determined the presence of blaOXA-96, blaCARB-2, and a novel blaOXA-421 g...

15 Production of OXA-23 carbapenemase is the most common reason for the increasing 16 carbapenem resistance in Acinetobacter spp. This study was conducted to reveal the genetic 17 basis of the blaOXA-23 dissemination in Acinetobacter spp. in China. A total of sixty-three 18 carbapenem-resistant OXA-23-producing Acinetobacter spp. isolates, representing different 19 backgrounds, were selected fr...