BACKGROUND In IVF-ICSI cycles with single embryo transfer (SET), embryo selection for transfer is of crucial importance. The present study aimed to define which embryo parameters might be related to the implantation potential of advanced blastocysts. METHODS Overall, in 203 cycles with SET, developmental characteristics of 93 implanted (group A) and 110 non-implanted (group B) advanced blasto...

OBJECTIVE To assess the effect of implementing different previtrification interventions on the postwarming DNA integrity of vitrified blastocysts. DESIGN Prospective in vitro study. SETTING Center for Reproductive Medicine laboratory in a tertiary hospital setting. ANIMALS A total of 70 expanded and 46 nonexpanded mouse blastocysts were used for the study. INTERVENTION(S) [1] Twenty-two...

This study compared the effect of using either CZB or TCM 199 media on both the development of 1-2 cell ovine embryos from superovulated ewes to the blastocyst stage (Experiment 1), and the hatching process of ovine blastocysts developed in vitro (Experiment 2). For the first 5 d, the CZB medium showed higher rates of embryo development than the TCM 199 medium (p < 0.001). The embryos reaching ...

Although improved outcomes of IVF after transfer of blastocysts has been reported (1, 2), it remains uncertain which blastocyst can develop into a viable fetus. At present, expansion of the blastocyst is the most practical selection criterion. However, if extended culture of embryos produces many expanded-stage blastocysts, it is not easy to decide which blastocysts to transfer (Fig. 1A). In ad...

The emergence of the mammalian blastocysts from their thick glycoprotein investment known as the zona pellucida is an important, but poorly understood, event in embryogenesis. In this paper, we demonstrate that peri-hatching blastocysts generate a considerably high quantum of an active oxyradical species for an extremely short period of time when compared to the pre-hatching (unhatched) and pos...

The effect of in vitro preimplantation exposure of mouse embryos to N-methyl-N-nitrosourea (MNU) on development subsequent to embryo transfer into pseudopregnant surrogate mothers has been investigated. The offspring developed from mouse blastocysts exposed to MNU or to solvent were examined carefully for 1 year after birth and then examined with X-rays and complete autopsies. Metaphase chromos...

The present study was conducted to examine the effects of culture systems and culture media on developmental competence and freezability of bovine embryos obtained by in vitro culture of in vitro matured and fertilized (IVM-IVF) oocytes. No significant difference was observed in the proportions of oocytes developed to blastocysts, the speed at which the oocytes reached the blastocyst stage and ...

Although selection of chromosomally normal embryos has the potential to improve outcomes for patients undergoing IVF, the clinical impact of aneuploidy screening by fluorescence in situ hybridization (FISH) has been controversial. There are many putative explanations including sampling error due to mosaicism, negative impact of biopsy, a lack of comprehensive chromosome screening, the possibili...

background: using cellular phone has rapidly increased all over the world. also, the concern on the possible health hazards of electromagnetic fields (emf) induced from cell phones to reproduction has been growing in many countries. the aim of this study was to assess the consequences and effects of exposure to the cell phone radiation on the quality and survival rates of preimplantation embryo...

The aim of the study was to analyse the toxicity, the osmolar and cryoprotective activity of ethylene glycol (ETG) in terms of survival rate (SR), cleavage rate (CR) and expanded blastocysts percentage (EBP) of mouse embryos. Early mouse embryos and blastocysts were slowly cooled with ETG, 1,2-propanediol (PROH) or glycerol, and thawed. The Van t'Hoff curve for 1.5 mol/l ETG showed recovery of ...