The ability to use dry blood spots (DBSs) on filter paper for the analysis of urea levels could be an important diagnostic tool for areas that have limited access to laboratory facilities. We developed a method for the extraction and quantification of urea from DBSs that were stored on 3M Whatman filter paper and investigated the effect of long-term storage on the level of urea in DBSs. DBSs of...

Most of the methods for the determination of urea in blood involve the use of the enzyme urease to hydrolyze the urea, since this reaction is specific. However, instead of crystalline urease (l), crude jack bean meal extracts are used. These extracts have been found to contain other enzymes besides urease, termed “ammonia-producing” enzymes, and their substrates, which may produce large quantit...

BACKGROUND Many metabolic changes develop in patients with chronic kidney disease which often necessitate frequent biochemical analysis of blood. Saliva analysis as an alternative to blood has many advantages. The aims of this study were to evaluate levels of salivary creatinine and urea in patients with chronic kidney disease in comparison to healthy individuals; to determine correlation betwe...

BACKGROUND The urea blood test (Ez-HBT) has been shown to compare favourably with the urea breath test in the diagnosis of active Helicobacter pylori infection. AIM To examine the performance characteristics of the Ez-HBT Helicobacter blood test in establishing success or failure of therapy in H. pylori-infected adults using the 13C urea breath test as the reference method. METHODS 13C urea...

On one side of the urea-secreting cells of the kidney there is blood and on the other side there is urine. The rate of urea excretion is the rate at which these cells transfer urea from blood to urine. Under these circumstances it seems probable that changes in the concentration of urea in the ,blood will have an effect on the rate of urea excretion. If the concentration of urea in the blood we...

Ammonia is the chief excretory product in fishes. However, non-availability of enough of water in the habitat, may lead to the formation of urea, in fishes. In the present study, the possible role of urea formation to avoid the toxicity of ammonia under water-restricted condition was tested in Channa gachua. Circulatory urea and ammonia were estimated in the blood of the fishes and glutamate de...

hydroalcoholic extract (70% ethanol extract) of tubers of momordica tuberosa cogn. (cucurbitaceae) was subjected to preliminary phytochemical screening by qualitative tests. nitric oxide scavenging activity was performed by griess reagent method. and nephroprotective activity was assessed in gentamicin, cisplatin and paracetamol induced renal damage in wistar rats (150-200 g) by standard method...

Svensgaard (1) using the micro urea method of Rehberg (2) compared the urea concentrations in blood from the arm vein with that of cutaneous blood from the lobe of the ear. In 29 of 32 analyses of the blood of normal fasting subjects she found the urea of the capillary blood higher thamn that of the venous blood. The average difference was 10 per cent of the venous urea content, but individual ...

The renal handling of urea has been studied by many investigators on a host of animals. It has been found that urea is actively secreted by certain species (Munro, '53; Forster, '54), reabsorbed by others (Smith, '31a, b), and in some the renal handling seems to be entirely passive (Shannen, '36). The aglomerular goose fish (Lophius piscatorius) is reportedly able to secrete urea actively from ...