hepatitis b virus (hbv) dna polymerase transactivated protein 1 (hbvdnaptp1) is a novel protein transactivated by hbv dna polymerase, screened by suppression subtractive hybridization technique (genbank accession no: ay450389). the biological function of hbvdnaptp1 was investigated in this study.we constructed a vector pcdna3.1 (-)/myc-his a-hbvdnaptp1 and used it to transfect acute monocytic l...

We compared the sensitivity of the polymerase chain-reaction (PCR) assay to that of slot-blot hybridization for detecting hepatitis B virus (HBV) DNA in the serum of 31 patients with chronic hepatitis. Of 14 chronic hepatitis patients positive for both HBV surface and HBV e antigens, 9 were positive for HBV DNA by slot-blot hybridization and all 14 by PCR. Also, of 9 patients positive for HBV s...

Several reports have been published on the use of polymorphisms found in the human hemoglobin genes as a means for prenatal diagnosis of sickle cell anemia. The disadvantages of this approach reside in its limited application and the need for family analysis. Here we report that, by use of restriction endonuclease Dde I and diazobenzyloxymethyl-paper transfer procedures, a direct analysis can b...

In this study, a modified method of the conventional RNA dot-blot hybridization was established, by replacing (32)P labels with CY5 labels and replacing nylon membranes with positive-charged glass slides, for detecting plant RNA viruses and a viroid. The modified RNA dot-blot hybridization method was named glass slide hybridization. The optimum efficiency of RNA binding onto the surfaces of act...

The size and sequence composition of virus-specific RNA extracted from the nuclei of mouse cells late during polyoma virus productive infection were studied by blot-hybridization analysis of 32P-labeled RNA fractionated on CH3HgOH/agarose gels. Viral RNA molecules between approximately 0.4 and 4 times the length of a complete transcript of the 5.4-kilobase circular viral DNA were found. Less th...

Resveratrol synthase (RS) is a key enzyme in (Res) synthesis pathway. RS genes have been transformed and expressed many plants microorganisms, play biological roles plant metabolism regulation. In this study, the full length sequence of resveratrol gene was into Cordyceps militaris by Agrobacterium tumefaciens infestation with 1179 bp. The integrated genome PCR, Southern blot hybridization, Nor...