Preparation and characterization of two new site-directed mutant copper-zinc superoxide dismutase proteins from Saccharomyces cerevisiae, i.e., His46Cys (H46C) and His120Cys (H120C), in which individual histidyl ligands in the copper-binding site were replaced by cysteine, are reported here. These two mutant CuZnSOD proteins may be described as type 2 (or normal) rather than type 1 (or blue) co...

Stellacyanin, or Rhus vernicijera blue protein, was purified from lac acetone powders. It is 20% carbohydrate and 20% hexosamine with the remainder being a polypeptide matrix of 108 amino acid residues in which is embedded a single cupric atom. The molecule contains no methionine or valine. Optical rotatory dispersion and optical and electron paramagnetic resonance (EPR) spectral studies show t...

Coordinate compounds of copper(II) are widely spread both as biological objects (metalloproteins and metallo-enzymes), and in engineering. Among the functions of the copper proteins are: the electron transfer involving the Cu(I)/Cu(II) couple; monoterminaloxidases, which form either water or hydrogen peroxide from dioxygen; oxygenases, which incorporate an oxygen atom into a substrate; superoxi...

In 1924, Hart, Elvehjem and coworkers demonstrated that copper is essential for erythropoiesis in most, if not all, mammalian species (1). Since that time many studies have been performed in attempts to elucidate the manner in which copper functions in erythropoiesis, but as yet this mechanism is not understood. Recent studies in this laboratory (2) have indicated that the amount of copper in t...

Solvent extraction system is one of the effective techniques used for separation of metal cations from aqueous solutions [1-8]. It is a suitable method for preventing and protecting environment from pollution. The extraction of copper(II) using carboxylic acids has been a subject of much work research [9-13]. Lauric acid was employed as an extractant for the separation of numerous cations, the ...

Solvent extraction system is one of the effective techniques used for separation of metal cations from aqueous solutions [1-8]. It is a suitable method for preventing and protecting environment from pollution. The extraction of copper(II) using carboxylic acids has been a subject of much work research [9-13]. Lauric acid was employed as an extractant for the separation of numerous cations, the ...

In 1924, Hart, Elvehjem and coworkers demonstrated that copper is essential for erythropoiesis in most, if not all, mammalian species (1). Since that time many studies have been performed in attempts to elucidate the manner in which copper functions in erythropoiesis, but as yet this mechanism is not understood. Recent studies in this laboratory (2) have indicated that the amount of copper in t...

In 1924, Hart, Elvehjem and coworkers demonstrated that copper is essential for erythropoiesis in most, if not all, mammalian species (1). Since that time many studies have been performed in attempts to elucidate the manner in which copper functions in erythropoiesis, but as yet this mechanism is not understood. Recent studies in this laboratory (2) have indicated that the amount of copper in t...

Laccases are green biocatalysts that possess attractive advantages for the treatment of resistant environmental pollutants and dye effluents. A putative laccase-like gene, laclK, encoding a protein of 29.3 kDa and belonging to the Cu-oxidase_4 superfamily, was cloned and overexpressed in Escherichia coli. The purified recombinant protein LaclK (LaclK) was able to oxidize typical laccase substra...