Group A rotaviruses were first detected in pigs suffering from diarrhea in 1975. It is generally accepted that multiple rotavirus strains are present in most if not all conventional swine herds. Rotavirus infections are very prevalent and are commonly associated with diarrhea in suckling and weaned pigs. Early studies also demonstrated that porcine rotaviruses are physically and serologically s...

A bovine rotavirus, NGRBg8, isolated from the feces of a calf with diarrhea in Nigeria was characterized by reverse transcription-PCR, nucleotide sequence analysis, and Northern blot hybridization. The nucleotide sequence of the VP7 gene of the strain was most closely related to that of a Nigerian human G8-serotype strain, HMG035 (99.9%). The NSP1 gene of strain NGRBg8 is highly related (99.4%)...

The epidemiology of animal rotaviruses infection and their association with illnesses in humans remains unknown Central America the world. In this study, we performed a genotyping rotavirus strains by RT-PCR for-G (VP7) P (VP4), samples diarrhea calves piglets, Honduras Panama. Mixed infections combinations genotypes G (VP4) were identified Honduras: 3 (4%) G2P [6], 2 (2%) [4], piglets: 4 (5%) ...

A rotavirus, designated as the H-1 strain, was isolated from a diarrheic foal in primary African green monkey kidney cells and MA104 cells. This cell culture-adapted strain hemagglutinated erythrocytes of human group O, rhesus monkeys, guinea pigs, and sheep. It was found to be similar, if not identical, to porcine rotaviruses (strains OSU, EE, and A-580) by plaque reduction neutralization and ...

We report the detection and molecular characterization of a rotavirus strain, 10733, isolated from the feces of a buffalo calf affected with diarrhea in Italy. Strain 10733 was classified as a P[3] rotavirus, as the VP8* trypsin cleavage product of the VP4 protein revealed a high amino acid identity (96.2%) with that of rhesus rotavirus strain RRV (P5B[3]), used as the recipient virus in the hu...

A virus (designated the Shintoku strain) which was morphologically indistinguishable from group A rotaviruses was detected in the feces of adult cows with diarrhea in Japan. The virus contained 11 segments of double-stranded RNA and had an electrophoretic migration pattern in polyacrylamide gels similar to that of other group C rotaviruses (4-3-2-2). Feces containing the bovine virus reacted wi...

Bovine rotaviruses isolated from beef and dairy herds in Argentina were serotyped by the immunoperoxidase focus reduction assay as previously described (G. Gerna, M. Battaglia, G. Milenesi, N. Passarani, E. Percivalle, and E. Cattaneo, Infect. Immun. 43:722-729, 1984). Three strains from beef herds were related to the UK and NCDV bovine rotavirus strains defined as serotype 6 (Y. Hoshino, R. G....

Antigenic and genomic properties of non-serotype 6 bovine rotaviruses isolated in Thailand and Japan were studied by cross-neutralization tests, nucleotide sequence determination of the VP7 gene, and RNA-RNA hybridization. Two Thai strains (61A and A44) were serologically related to a Japanese isolate KK3 which has been assigned to serotype 10. In contrast, strain A5 was found to be antigenical...

Hybridomas secreting monoclonal antibodies (MAbs) to a group A rotavirus (GAR) were developed by fusion of myeloma cell (SP2/0) with spleen cells of BALB/c mice immunized with semi-purified bovine rotavirus (CR129, G10P11). Two of these rotavirus-specific monoclonal antibodies were further characterized by immunoblotting and also tested for their reactivity with rotaviruses of bovine, equine an...