Abstract: The aim of the present study was to produce a polyclonal antibody against bovine serum albumin (BSA) conjugated with artemisinin. To gain an immunogenic character of artemisinin, a carboxyl group was added to it using mixed anhydride method. Then, the reactive compound of artemisinin was conjugated with BSA. The BSA+artemisinin were injected to white female New Zealand rabbits for two...

Background and objectives Determination of different grain protein fractions in wheat cultivars is an important task analyzing bread baking quality. In many laboratories, the Bradford assay used to determine concentrations solutions. any (including Bradford), ideal use as a standard purified being assayed. absence such absolute reference, another must be selected relative bovine serum albumin (...

Objective: The study ondrug–protein interactions is an important field of interest because the prospective unraveling drug action mechanisms and possibility designing novel medicines. Bovine serum albumin (BSA) has been studied extensively its structural homology with human (HSA). objective work was to interaction between β-sitosterol Luteolin–7-glucoside bovine investigated by molecular dockin...

Light up, light up: The fluorimetric detection of different biomacromolecules is accomplished with 3-(4-N,N-dimethylaminophenylnaphtho[1,2-b]quinolizinium as a fluorescent probe. This molecule shows light-up effect in the presence DNA, RNA and bovine serum albumin (BSA) emission energies, intensities, lifetimes. These effects may be used for differentiation between cell compartments.

Electrochemical characterization and detection of protonated dibucaine (DIC+) at microinterface array across water|1,6-dichlorohexane were performed using cyclic voltammetry (CV) differential pulse (DPV). Some thermodynamic parameters dibucaine, such as the standard transfer potential, Gibbs energy partition coefficient, estimated by CV. In addition to analytical parameters, impact bovine serum...

The effect of arsenic on the binding metronidazole (an anti-amoebic agent) to bovine serum albumin (BSA) was studied by equilibrium dialysis (ED) method in order justify nature capability drug and BSA. There decrease concentration due addition arsenic. But free increased with increasing when only BSA present. result obtained no added 41% whereas this release 7% an from 0.5x10-5 10x10-5M. 36% 71...

By coupling 5-Methyl-cytidine to bovine serum albumin, a conjugate containing 18 mol of 5-Methyl-cytidine per mol of BSA was prepared. Antibodies to this conjugate were produced by immunization of rabbits. Specificity of the antibody was assessed by gel diffusion in agar containing excessive amounts of the carrier BSA. A slight cross-reactivity with cytidine was eliminated by adsorption on the ...

The binding characteristics of indigotin with human serum albumin (HSA) and bovine serum albumin (BSA) have been investigated by various spectroscopic techniques. Spectroscopic analysis revealed that the quenching mechanism between indigotin and HSA/BSA belonged to the static quenching. The displacement experiments suggested that indigotin primarily bound to tryptophan residues on proteins with...

In this paper, the quartz crystal microbalance with dissipation monitoring (QCM-D) was used to investigate hydrophobicity and binding strength (KD) for 10 different drugs interacting human serum albumin (HSA). Quantitative structure activity relationship (QSAR) analysis determine between drug (ClogP) HSA log(1/KD). The results are compared prior knowledge on bovine (BSA) binding. We demonstrate...

interaction of drugs with serum albumin, the most abundant protein in plasma, has a great significance in pharmaceutical sciences. it can affect the biological activity, toxicity, and pharmacokinetics of drugs and design of dosages. determination of the impact of chemical modifications of albumin and its structural changes upon interaction with drugs are very important when drugs bind with albu...