in this syudy a continuous bovine kidney cell line derived from a primary bovine kidney cells was established for the first time in iran. the cells were originating from two-day-old normal male calf of holstein breed. the cell cultures were continuously passaged following complete proliferation of primary cells. the specific properties or characteristics of the cell were defined using cytogene...

Background: In reproductive biology, testicular organoids can be used to treat infertility and study development spermatogonial stem cells (SSCs) differentiation. Generating organoid from primary is challenging. this study, were created using human evaluated the apoptotic gene expression hormone secretion profiles of organoids. Materials Methods: Primary isolated 2-step enzymatic digestion thre...

BACKGROUND Extensive research is ongoing to empower cancer survivors to have biological parenthood. For this, sperm are cryopreserved prior to therapy and in younger children testicular biopsies are cryopreserved with a hope to mature the germ cells into sperm later on for assisted reproduction. In addition, lot of hope was bestowed on pluripotent embryonic and induced pluripotent stem cells to...

Crude preparations of bovine testicular hyaluronidase (1) depolymerize both hyaluronic acid (HA) and chondroitinsulfuric acid (CSA). Since a series of enzyme preparations with a wide range (loo-fold) of purity shows a constant ratio of activities toward both substrates when examined by a turbidimetric method of assay, the conclusion was drawn that both activities were most likely assignable to ...

In 13 and 14-day old fetal rat ovaries maintained 3 to 10 days in organ culture, purified bovine anti-Müllerian hormone (AMH) (1.5 to 3 micrograms/ml) induced a characteristic freemartin effect. Gonadal volume and germ cell number were significantly reduced, compared to control ovaries cultured in anhormonal medium, and epithelial cells with large clear cytoplasm linked by interdigitations diff...

Introduction. Male infertility is one of the main causes forced childlessness couples around world. Currently, a limited number drugs for treatment male are available on market. Biologically active supplements (dietary supplements) containing L-carnitine often used in complex therapy. The results determining therapeutic activity new drug, peptide obtained from cattle testes (PP-R-001) and compa...

Background: Aging in the male reproductive system can cause testicular degeneration. Nowadays, regenerative medicine is a developing domain. This study conducted to describe potential effects of dried bovine placenta supplementation on percentage abnormal spermatozoa, density seminiferous tubules luminal content and Leydig cells counts D-galactose-induced aging rats. Methods: A total 15 healthy...

objective: electroporation can be a highly efficient method for introducing the foreign genetic materials into the targeted cells for transient and/or permanent genetic modification. considering the application of this technique as a very efficient method for drug, oligonucleotide, antibody and plasmid delivery for clinical applications and production of transgenic animals, the present study ai...

background: long term alcohol use has been implicated in men with sexual disorders including suppression of testosterone levels as well as testicular morphological changes. objective: this research investigated the ability of telfairia occidentalis (t.o.) to attenuate the damaging effects of alcohol on the testicular parameters. materials and methods: thirty male sprague-dawley rats, 170-190 gr...

The growth of poliomyelitis virus, Type 2, Yale-SK strain, in cultures of monkey testicular tissue was observed to occur in discrete cycles. Growth curves showed that each cycle was composed of (a) an initial lag phase when little or no virus was released from the cells, (6) a phase of viral production, and (c) a plateau which represented a decrement in the rate of viral production. This patter...