The denatured catalytic polypeptide of (Na+,K+)-ATPase of goldfish brain was purified and identified as the 32P-labeled phosphoprotein. The protein served as immunogen for the preparation of rabbit antisera for immunohistochemical application t o goldfish tissue sections, using t h e peroxidase-antiperoxidase indirect method. Labeling in brain cross-sections appears primarily in fibers of the o...

Five different staining methods were done on the sheep brain compared and best method was employed to stain human brain, Alstons is found be among five method, which shows excellent differentiation of grey white matter. Alston’s has low shrinkage percentage time taken for this procedure around 8 hrs 30 min. When with unstained section stained show pronounced Learning neuroanatomy wet difficult ...

Mitochondrial dysfunction has been implicated in many neurological disorders that only develop or are much more severe in adults, yet no methodology exists that allows for medium-throughput functional mitochondrial analysis of brain sections from adult animals. We developed a technique for quantifying mitochondrial respiration in acutely isolated adult rat brain sections with the Seahorse XF An...

Optogenetic constructs have revolutionized modern neuroscience, but the ability to accurately and efficiently assess their expression in the brain and associate it with prior functional measures remains a challenge. High-resolution imaging of thick, fixed brain sections would make such post-hoc assessment and association possible; however, thick sections often display autofluorescence that limi...

We made an MR-compatible brain slice holder that ensures that the images have the same location and orientation as the subsequent histologic sections. This device encloses the unfixed tissues, thus reducing the danger of exposure to pathogens. MR imaging time is saved by scanning selected slices rather than the whole brain; this technique is especially useful when brain sections at varying angl...

We report a fatal case of encephalitis caused by Acanthamoeba in a 24-year-old woman from India with systemic lupus erythematosus. Diagnosis was made by identification of amebas in brain sections by immunofluorescence analysis and confirmed by demonstrating Acanthamoeba mitochondrial 16S rRNA gene DNA in brain tissue sections.

We describe an enzyme histochemical technique for the simultaneous demonstration of acetylcholinesterase (AChE) and monoamine oxidase (MAO) (Types A, B, or A+B) in fixed-frozen sections. Several regions in the mesencephalon and brainstem were examined for both somatic and neuropil labeling. The results obtained are equivalent or superior to those obtained using previous methods for the individu...

Brain-invasive meningiomas have an adverse prognosis, so it is important to detect and correctly evaluate brain invasion by light microscopy. Furthermore, the underlying biological mechanisms responsible for brain-invasive growth are incompletely understood. The primary aim of this study was to identify immunohistochemical markers that could improve identification and evaluation of brain invasi...